|
| Status |
Public on Sep 24, 2023 |
| Title |
PAECs, hypoxia condition, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
PAECs, hypoxia condition
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Pulmonary artery endothelial cells (PAECs)
stress: hypoxia
|
| Treatment protocol |
Pulmonary artery endothelial cells (PAECs) were exposed to normoxia condition (21% O2, 5% CO2, 74% N2) or hypoxia condition (3% O2, 5% CO2, 92% N2) (n=3 for each group).PAECs were harvested after 24 hours and performed to further analysis.
|
| Growth protocol |
Cell growth was arrested by incubating in 2% serum and GS-free ECM for 6 h under normoxia, then growth-arrested cells were incubated with low-serum (5% FBS) and GS-free ECM under normoxia or hypoxia for 24 h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
cDNAs of samples from normoxia and hypoxia group were hybridized to Affymetrix Clariom D human GeneChip® arrays (Affymetrix, America) according to the User Manuals. Affymetrix® Expression Console Software (version 1.2.1) was used for microarray analysis. Raw data (CEL files) were normalized at transcript level using robust multiaverage method (RMA workflow). Median summarization of transcript expressions was calculated. Gene-level data was then filtered to include only those probe sets that are in the ‘core’ metaprobe list, which represent RefSeq genes.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-8 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10ug of cRNA were hybridized for 16 hr at 45℃ on [MTA-1_0] Affymetrix Mouse Transcriptome Array 1.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Genechips were scanned by GeneChip® Scanner 3000.
|
| Data processing |
The data were analyzed with Expression Console™, Version 1.3.1.
|
| |
|
| Submission date |
Sep 19, 2023 |
| Last update date |
Sep 24, 2023 |
| Contact name |
李 玉琳 |
| Organization name |
Beijing Institute of Heart Lung and Blood Vessel Diseases
|
| Street address |
2 Anzhen Rd, Chaoyang District,
|
| City |
Beijing |
| ZIP/Postal code |
100029 |
| Country |
China |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE243567 |
Expression data from pulmonary artery endothelial cell after exposure to hypoxia for 24 hours |
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