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Sample GSM7791177 Query DataSets for GSM7791177
Status Public on Sep 24, 2023
Title PAECs, hypoxia condition, rep2
Sample type RNA
Source name PAECs, hypoxia condition
Organism Homo sapiens
Characteristics cell type: Pulmonary artery endothelial cells (PAECs)
stress: hypoxia
Treatment protocol Pulmonary artery endothelial cells (PAECs) were exposed to normoxia condition (21% O2, 5% CO2, 74% N2) or hypoxia condition (3% O2, 5% CO2, 92% N2) (n=3 for each group).PAECs were harvested after 24 hours and performed to further analysis.
Growth protocol Cell growth was arrested by incubating in 2% serum and GS-free ECM for 6 h under normoxia, then growth-arrested cells were incubated with low-serum (5% FBS) and GS-free ECM under normoxia or hypoxia for 24 h.
Extracted molecule total RNA
Extraction protocol cDNAs of samples from normoxia and hypoxia group were hybridized to Affymetrix Clariom D human GeneChip® arrays (Affymetrix, America) according to the User Manuals. Affymetrix® Expression Console Software (version 1.2.1) was used for microarray analysis. Raw data (CEL files) were normalized at transcript level using robust multiaverage method (RMA workflow). Median summarization of transcript expressions was calculated. Gene-level data was then filtered to include only those probe sets that are in the ‘core’ metaprobe list, which represent RefSeq genes.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-8 ug total RNA
Hybridization protocol Following fragmentation, 10ug of cRNA were hybridized for 16 hr at 45℃ on [MTA-1_0] Affymetrix Mouse Transcriptome Array 1.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol Genechips were scanned by GeneChip® Scanner 3000.
Data processing The data were analyzed with Expression Console™, Version 1.3.1.
Submission date Sep 19, 2023
Last update date Sep 24, 2023
Contact name 李 玉琳
Organization name Beijing Institute of Heart Lung and Blood Vessel Diseases
Street address 2 Anzhen Rd, Chaoyang District,
City Beijing
ZIP/Postal code 100029
Country China
Platform ID GPL23126
Series (1)
GSE243567 Expression data from pulmonary artery endothelial cell after exposure to hypoxia for 24 hours

Data table header descriptions
VALUE RMA signal intensity

Data table
AFFX-BkGr-GC03_st 6.61718
AFFX-BkGr-GC04_st 5.85173
AFFX-BkGr-GC05_st 5.55434
AFFX-BkGr-GC06_st 4.98833
AFFX-BkGr-GC07_st 4.09315
AFFX-BkGr-GC08_st 3.57657
AFFX-BkGr-GC09_st 3.24698
AFFX-BkGr-GC10_st 3.08477
AFFX-BkGr-GC11_st 2.71068
AFFX-BkGr-GC12_st 2.59932
AFFX-BkGr-GC13_st 2.28103
AFFX-BkGr-GC14_st 2.14121
AFFX-BkGr-GC15_st 2.03653
AFFX-BkGr-GC16_st 1.99796
AFFX-BkGr-GC17_st 2.02438
AFFX-BkGr-GC18_st 2.0158
AFFX-BkGr-GC19_st 2.13574
AFFX-BkGr-GC20_st 2.2341
AFFX-BkGr-GC21_st 2.24116
AFFX-BkGr-GC22_st 2.44175

Total number of rows: 138745

Table truncated, full table size 3533 Kbytes.

Supplementary file Size Download File type/resource
GSM7791177_H_2.CEL.gz 20.4 Mb (ftp)(http) CEL
GSM7791177_H_2.sst-rma-gene-full.chp.gz 885.2 Kb (ftp)(http) CHP

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