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Sample GSM7792409 Query DataSets for GSM7792409
Status Public on Jan 10, 2024
Title LTBI_10408_pre-plasma IgM
Sample type protein
 
Source name pre-plasma
Organism Macaca fascicularis
Characteristics infection states: Asymptomatic controlled Mtb infection
Treatment protocol BALF dilted (1:8) or plasma diluted (1:100)
Growth protocol N/A
Extracted molecule protein
Extraction protocol Plasma was obtained from peripheral blood samples and the BAL procedure was performed as previously described via direct visualization of the airway and lavage of ~ 40 ml normal saline (0.9%). Animal protocols and procedures were approved by the University of Pittsburgh’s Institutional Animal Care and Use Committee before the collection of samples.
Label Anti-human IgM DyLight™ 649
Label protocol Anti-human IgM (mu chain) (GOAT) Antibody DyLight™ 649 Conjugated - 609-143-007 (Rockland)
 
Hybridization protocol Slides with 8 arrays on each were blocked with 3% BSA (GEMINi Bio-Products, standard grade) in PBS at 4°C overnight, washed with 0.1 % PBS-T, and then incubated with BALF (1:8) or plasma (1:100), diluted in 0.1% PBST with 3% BSA, for 4 hrs at 37°C. After washing, bound antibodies were detected by Cy3 Goat Anti-Human Serum IgA (Jackson ImmunoResearch Labs Cat# 109-165-011, RRID:AB 2337721) and Alexa 647 Goat Anti-Human IgG (Jackson ImmunoResearch Labs Cat# 109-605-008, RRID:AB_2337882. Slides were then washed with PBST, spun dry in a centrifuge, and scanned.
Scan protocol The slides were scanned using the GenePix 4000 Microarray scanner system. Scanned images were analyzed with by the image-processing software G\enePix Pro 7.3.0.0 to analyze the images and measure the median pixel intensity (MPI).
Description IgM
Data processing The median pixel intensity of each feature was background subtracted. A minimum value of pixel intensity (400) was determined by the median pixel intensity of the spots with low quality, which was determined by the software quality control score for each spot depending on signal-to-noise ratio and spot shape. The scanned images were visually inspected. The spots with obvious local artifacts were excluded from analysis. The array features were printed in triplicate. The final data were averaged from the triplicates.
 
Submission date Sep 20, 2023
Last update date Jan 10, 2024
Contact name Jacqueline M. Achkar
E-mail(s) Jacqueline.achkar@einsteinmed.edu
Phone 7184302971
Organization name Albert Einstein College of Medicine
Street address 1300 Morris Park Ave
City Bronx
State/province NY
ZIP/Postal code 10461
Country USA
 
Platform ID GPL27885
Series (2)
GSE243657 Mucosal and systemic antigen-specific antibody responses correlate with protection against active tuberculosis in nonhuman primates (LAM30 IgM)
GSE243663 Mucosal and systemic antigen-specific antibody responses correlate with protection against active tuberculosis in nonhuman primates

Data table header descriptions
ID_REF
VALUE normalized (scaled) signal count data.

Data table
ID_REF VALUE
1 2329
2 1246.666667
3 1125.333333
4 1045.666667
5 961
6 1058.333333
7 1225.5
8 1079.666667
9 1140
10 1221
11 1271.666667
12 1366.666667
13 1620.5
14 1297.333333
15 2298.5
16 1434.666667
17 1215
18 992
19 2544.666667
20 1477

Total number of rows: 30

Table truncated, full table size <1 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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