|
| Status |
Public on Oct 02, 2023 |
| Title |
Patient#5 |
| Sample type |
RNA |
| |
|
| Source name |
epithelial ovarian cancer
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: ovary
Stage: 3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Nanostring technology, total RNA was extracted from FFPE samples by using Maxwell RSC RNA FFPE Kit and Maxwell RSC Instrument (Promega), according to manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
NanoString target-specific oligonucleotide probe pairs, fluorescently-labeled specific Reporter Tags, and a biotinylated universal Capture Tag were used
|
| |
|
| Hybridization protocol |
hybridization was performed at 65°C for 16h
|
| Scan protocol |
data were collected using nCounter Digital Analyzer at the standard resolution of 280 fields of view (fov)
|
| Data processing |
Raw counts were extracted from the nSolver analysis software 4.0 and raw RCC files were analysed using nSolver Software and Advaced Analysis Module
|
| |
|
| Submission date |
Sep 21, 2023 |
| Last update date |
Oct 02, 2023 |
| Contact name |
Chiara Balestrieri |
| E-mail(s) |
balestrieri.c@gmail.com
|
| Organization name |
IRCCS San Raffaele Scientific Institute
|
| Department |
Center for Omics Sciences
|
| Street address |
Via Olgettina 58
|
| City |
Milan |
| ZIP/Postal code |
20132 |
| Country |
Italy |
| |
|
| Platform ID |
GPL31236 |
| Series (1) |
| GSE243751 |
Epithelial Ovarian Cancer is infiltrated by activated effector T cells co-expressing CD39, PD-1, TIM-3, CD137 and interacting with cancer cells and myeloid cells |
|
