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Sample GSM7795301 Query DataSets for GSM7795301
Status Public on Oct 02, 2023
Title Patient#20
Sample type RNA
 
Source name epithelial ovarian cancer
Organism Homo sapiens
Characteristics tissue: ovary
Stage: 3
Extracted molecule total RNA
Extraction protocol Nanostring technology, total RNA was extracted from FFPE samples by using Maxwell RSC RNA FFPE Kit and Maxwell RSC Instrument (Promega), according to manufacturer’s instructions.
Label biotin
Label protocol NanoString target-specific oligonucleotide probe pairs, fluorescently-labeled specific Reporter Tags, and a biotinylated universal Capture Tag were used
 
Hybridization protocol hybridization was performed at 65°C for 16h
Scan protocol data were collected using nCounter Digital Analyzer at the standard resolution of 280 fields of view (fov)
Data processing Raw counts were extracted from the nSolver analysis software 4.0 and raw RCC files were analysed using nSolver Software and Advaced Analysis Module
 
Submission date Sep 21, 2023
Last update date Oct 02, 2023
Contact name Chiara Balestrieri
E-mail(s) balestrieri.c@gmail.com
Organization name IRCCS San Raffaele Scientific Institute
Lab Center for Omics Sciences
Street address Via Olgettina 58
City Milan
ZIP/Postal code 20132
Country Italy
 
Platform ID GPL31236
Series (1)
GSE243751 Epithelial Ovarian Cancer is infiltrated by activated effector T cells co-expressing CD39, PD-1, TIM-3, CD137 and interacting with cancer cells and myeloid cells

Supplementary file Size Download File type/resource
GSM7795301_Patient_20.RCC.gz 9.1 Kb (ftp)(http) RCC

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