|
| Status |
Public on Sep 26, 2023 |
| Title |
swi6-delta set1-delta _9me2_input_rep1_ectopic |
| Sample type |
SRA |
| |
|
| Source name |
fission yeast
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
genotype: swi6-delta set1-delta
chip antibody: 9me2
treatment: input
cell type: fission yeast
|
| Growth protocol |
YEA, grown to log phase at 32C
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were fixed with 1% formaldehyde for 15 minutes. Cells were lysed with bead beating.
ChIP-seq libraries were prepared with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina. 1 ng of DNA was used as starting material.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
sequences were trimmed with Trimmomatic, aligned with BWA, and normalized using bamCompare with subtraction of input and RPKM
Supplementary files format and content: bigwig files from bamCompare
|
| |
|
| Submission date |
Sep 25, 2023 |
| Last update date |
Sep 27, 2023 |
| Contact name |
Melissa Seman |
| E-mail(s) |
mseman@brandeis.edu
|
| Organization name |
Brandeis University
|
| Street address |
415 South St
|
| City |
Waltham |
| ZIP/Postal code |
02453 |
| Country |
USA |
| |
|
| Platform ID |
GPL20584 |
| Series (1) |
| GSE244017 |
Uncoupling the distinct functions of HP1 proteins during heterochromatin establishment and maintenance |
|
| Relations |
| BioSample |
SAMN37538139 |
| SRA |
SRX21883786 |
