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Sample GSM7804976 Query DataSets for GSM7804976
Status Public on Sep 26, 2023
Title WT_9me3_input_rep1_ectopic
Sample type SRA
 
Source name fission yeast
Organism Schizosaccharomyces pombe
Characteristics genotype: WT
chip antibody: 9me3
treatment: input
cell type: fission yeast
Growth protocol YEA, grown to log phase at 32C
Extracted molecule genomic DNA
Extraction protocol Cells were fixed with 1% formaldehyde for 15 minutes. Cells were lysed with bead beating.
ChIP-seq libraries were prepared with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina. 1 ng of DNA was used as starting material.
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina NextSeq 500
 
Data processing sequences were trimmed with Trimmomatic, aligned with BWA, and normalized using bamCompare with subtraction of input and RPKM
Supplementary files format and content: bigwig files from bamCompare
 
Submission date Sep 25, 2023
Last update date Sep 27, 2023
Contact name Melissa Seman
E-mail(s) mseman@brandeis.edu
Organization name Brandeis University
Street address 415 South St
City Waltham
ZIP/Postal code 02453
Country USA
 
Platform ID GPL20584
Series (1)
GSE244017 Uncoupling the distinct functions of HP1 proteins during heterochromatin establishment and maintenance
Relations
BioSample SAMN37538114
SRA SRX21883835

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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