|
Status |
Public on Sep 26, 2023 |
Title |
WT_9me3_input_rep1_ectopic |
Sample type |
SRA |
|
|
Source name |
fission yeast
|
Organism |
Schizosaccharomyces pombe |
Characteristics |
genotype: WT chip antibody: 9me3 treatment: input cell type: fission yeast
|
Growth protocol |
YEA, grown to log phase at 32C
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Cells were fixed with 1% formaldehyde for 15 minutes. Cells were lysed with bead beating. ChIP-seq libraries were prepared with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina. 1 ng of DNA was used as starting material.
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
sequences were trimmed with Trimmomatic, aligned with BWA, and normalized using bamCompare with subtraction of input and RPKM Supplementary files format and content: bigwig files from bamCompare
|
|
|
Submission date |
Sep 25, 2023 |
Last update date |
Sep 27, 2023 |
Contact name |
Melissa Seman |
E-mail(s) |
mseman@brandeis.edu
|
Organization name |
Brandeis University
|
Street address |
415 South St
|
City |
Waltham |
ZIP/Postal code |
02453 |
Country |
USA |
|
|
Platform ID |
GPL20584 |
Series (1) |
GSE244017 |
Uncoupling the distinct functions of HP1 proteins during heterochromatin establishment and maintenance |
|
Relations |
BioSample |
SAMN37538114 |
SRA |
SRX21883835 |