|
Status |
Public on Oct 03, 2023 |
Title |
2-week LNAME/HFD - 2524 |
Sample type |
SRA |
|
|
Source name |
Whole Lung
|
Organism |
Mus musculus |
Characteristics |
tissue: Whole Lung genotype: C57BL6/J treatment: L-NAME/HFD for 2 weeks
|
Treatment protocol |
Mice underwent treatment with either L-NAME (0.5 g/L) in the water and high fat diet (60% lipid content) for 2 and 5 weeks, compared to 0 week controls.
|
Extracted molecule |
total RNA |
Extraction protocol |
After sacrifice, lungs were immediately taken and flash frozen. RNA was isolated using Rneasy kit (Qiagen). RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
Partek Flow platform used for data processing Tophat 2 was used to align reads to the mm10 genome build Reads were quantified to mm10 using the Partek E/M annotation model Reads were normalized to counts per million (CPM) with 0.0001 added to the result. Assembly: mm10 Supplementary files format and content: Text file including CPM for each sample (in columns) and gene (in rows)
|
|
|
Submission date |
Sep 28, 2023 |
Last update date |
Oct 03, 2023 |
Contact name |
James West |
E-mail(s) |
j.west@vanderbilt.edu
|
Organization name |
Vanderbilt University
|
Department |
Medicine
|
Lab |
Pulmonary
|
Street address |
4200 E 9TH Ave, Box B133
|
City |
Nashville |
State/province |
TN |
ZIP/Postal code |
37201 |
Country |
USA |
|
|
Platform ID |
GPL13112 |
Series (1) |
GSE244304 |
Myeloid Cell Derived IL1b Contributes to Pulmonary Vascular Remodeling in Heart Failure with Preserved Ejection Fraction |
|
Relations |
BioSample |
SAMN37596237 |
SRA |
SRX21924236 |