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Sample GSM7813220 Query DataSets for GSM7813220
Status Public on Oct 03, 2023
Title 5-week LNAME/HFD - 2532
Sample type SRA
 
Source name Whole Lung
Organism Mus musculus
Characteristics tissue: Whole Lung
genotype: C57BL6/J
treatment: L-NAME/HFD for 5 weeks
Treatment protocol Mice underwent treatment with either L-NAME (0.5 g/L) in the water and high fat diet (60% lipid content) for 2 and 5 weeks, compared to 0 week controls.
Extracted molecule total RNA
Extraction protocol After sacrifice, lungs were immediately taken and flash frozen. RNA was isolated using Rneasy kit (Qiagen).
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing Partek Flow platform used for data processing
Tophat 2 was used to align reads to the mm10 genome build
Reads were quantified to mm10 using the Partek E/M annotation model
Reads were normalized to counts per million (CPM) with 0.0001 added to the result.
Assembly: mm10
Supplementary files format and content: Text file including CPM for each sample (in columns) and gene (in rows)
 
Submission date Sep 28, 2023
Last update date Oct 03, 2023
Contact name James West
E-mail(s) j.west@vanderbilt.edu
Organization name Vanderbilt University
Department Medicine
Lab Pulmonary
Street address 4200 E 9TH Ave, Box B133
City Nashville
State/province TN
ZIP/Postal code 37201
Country USA
 
Platform ID GPL13112
Series (1)
GSE244304 Myeloid Cell Derived IL1b Contributes to Pulmonary Vascular Remodeling in Heart Failure with Preserved Ejection Fraction
Relations
BioSample SAMN37596231
SRA SRX21924242

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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