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Sample GSM781653 Query DataSets for GSM781653
Status Public on Jul 18, 2017
Title DGM-00529_Tr
Sample type RNA
Source name Trachea
Organism Homo sapiens
Characteristics smoking status: NS
Extracted molecule total RNA
Extraction protocol Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-2 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Submission date Aug 17, 2011
Last update date Jul 18, 2017
Contact name Yael Strulovici-Barel
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
Platform ID GPL570
Series (2)
GSE31449 Positional Identity of Branching-associated HOXB2 and B3 Gene Expression is Maintained by Adult Human Airway Epithelial Progenitor Cells
GSE64614 Distal-to-Proximal Re-patterning of Small Airway Epithelium in Smoking-associated Chronic Obstructive Pulmonary Disease

Data table header descriptions
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)

Data table
AFFX-BioB-5_at 565.638 P 0.00320999
AFFX-BioB-M_at 396.765 P 0.000389797
AFFX-BioB-3_at 344.948 P 0.00179591
AFFX-BioC-5_at 966.804 P 0.000169227
AFFX-BioC-3_at 1075.89 P 4.42873e-05
AFFX-BioDn-5_at 1854.54 P 0.00010954
AFFX-BioDn-3_at 5107.5 P 0.000169227
AFFX-CreX-5_at 9169.72 P 4.42873e-05
AFFX-CreX-3_at 13901.5 P 5.16732e-05
AFFX-DapX-5_at 13.814 A 0.659339
AFFX-DapX-M_at 103.01 A 0.216524
AFFX-DapX-3_at 18.3851 A 0.897855
AFFX-LysX-5_at 63.9569 A 0.354453
AFFX-LysX-M_at 22.7202 A 0.712257
AFFX-LysX-3_at 57.5511 A 0.123572
AFFX-PheX-5_at 14.9664 A 0.891021
AFFX-PheX-M_at 21.9391 A 0.876448
AFFX-PheX-3_at 84.3452 A 0.39692
AFFX-ThrX-5_at 21.5978 A 0.617401
AFFX-ThrX-M_at 73.0301 A 0.41138

Total number of rows: 54675

Table truncated, full table size 1625 Kbytes.

Supplementary file Size Download File type/resource
GSM781653.CEL.gz 8.3 Mb (ftp)(http) CEL
GSM781653.CHP.gz 13.3 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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