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Sample GSM781662 Query DataSets for GSM781662
Status Public on Jul 18, 2017
Title DGM-00685_Tr
Sample type RNA
Source name Trachea
Organism Homo sapiens
Characteristics smoking status: NS
Extracted molecule total RNA
Extraction protocol Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-2 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Submission date Aug 17, 2011
Last update date Jul 18, 2017
Contact name Yael Strulovici-Barel
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
Platform ID GPL570
Series (2)
GSE31449 Positional Identity of Branching-associated HOXB2 and B3 Gene Expression is Maintained by Adult Human Airway Epithelial Progenitor Cells
GSE64614 Distal-to-Proximal Re-patterning of Small Airway Epithelium in Smoking-associated Chronic Obstructive Pulmonary Disease

Data table header descriptions
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)

Data table
AFFX-BioB-5_at 619.716 P 0.000753643
AFFX-BioB-M_at 726.737 P 4.42873e-05
AFFX-BioB-3_at 445.984 P 0.000169227
AFFX-BioC-5_at 1500.99 P 9.4506e-05
AFFX-BioC-3_at 1974.73 P 4.42873e-05
AFFX-BioDn-5_at 3433.43 P 4.42873e-05
AFFX-BioDn-3_at 7916.63 P 7.00668e-05
AFFX-CreX-5_at 17173.2 P 4.42873e-05
AFFX-CreX-3_at 22490.5 P 4.42873e-05
AFFX-DapX-5_at 41.839 A 0.287743
AFFX-DapX-M_at 49.9184 A 0.52976
AFFX-DapX-3_at 6.22624 A 0.984825
AFFX-LysX-5_at 7.5515 A 0.921998
AFFX-LysX-M_at 10.6318 A 0.910522
AFFX-LysX-3_at 32.7401 A 0.313723
AFFX-PheX-5_at 31.2366 A 0.425962
AFFX-PheX-M_at 5.93071 A 0.910522
AFFX-PheX-3_at 18.2041 A 0.52976
AFFX-ThrX-5_at 7.33409 A 0.794268
AFFX-ThrX-M_at 27.975 A 0.645547

Total number of rows: 54675

Table truncated, full table size 1636 Kbytes.

Supplementary file Size Download File type/resource
GSM781662.CEL.gz 8.4 Mb (ftp)(http) CEL
GSM781662.CHP.gz 13.6 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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