|
Status |
Public on Dec 01, 2023 |
Title |
N2 ctrl RNAi rep 3 |
Sample type |
SRA |
|
|
Source name |
Whole worm
|
Organism |
Caenorhabditis elegans |
Characteristics |
tissue: Whole worm developmental stage: Young Adult strain: N2 genotype: Wildtype treatment: empty vector RNAi
|
Growth protocol |
Embryos were hatched on NGM plates seeded with RNAi vectors and allowed to develop 72 hrs
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Worms were collected in Trizol (Invitogen) and purified using TURBO Dnase(Ambion) followed by Rneasy extraction kit (Qiagen) Poly-A selected mRNA library preparation was done using KAPA HyperPrep stranded library preparation kit
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
NextSeq 2000 |
|
|
Description |
N2_ctrl_rep3
|
Data processing |
Reads were aligned to C. elegans reference genome using STAR (v.2.7.10b) Differential gene expression analysis was performed using Feature counts and DeSeq2 Assembly: C. elegans WS275 Supplementary files format and content: .csv files include DeSeq2 analysis with normalized read counts
|
|
|
Submission date |
Oct 04, 2023 |
Last update date |
Dec 01, 2023 |
Contact name |
Emily Ann Erdmann |
E-mail(s) |
emierdma@iu.edu
|
Organization name |
Indiana University
|
Department |
Biology
|
Lab |
Hundley
|
Street address |
1001 E Third Street
|
City |
BLOOMINGTON |
State/province |
IN |
ZIP/Postal code |
47405 |
Country |
USA |
|
|
Platform ID |
GPL32326 |
Series (1) |
GSE244602 |
A high-throughput screen identifies RNA Binding Proteins that affect fertility in C. elegans and reveals a functional relationship between ADR-2 and SQD-1 |
|
Relations |
BioSample |
SAMN37674261 |
SRA |
SRX21986532 |