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Sample GSM7822395 Query DataSets for GSM7822395
Status Public on Apr 16, 2024
Title W0Pol1
Sample type SRA
Source name JJN3 human multiple myeloma cell line
Organism Homo sapiens
Characteristics treatment: DMSO
antibody: CST 4H8 #2629 (RPB1)
cell line: JJN3
cell type: multiple myeloma
Treatment protocol Cells were treated with DMSO or WNK463 (5μM) for 4 h
Growth protocol JJN3 cells were cultured in 40% DMEM 40% IMDM, and 20% FBS in 37°C supplemented with 10% CO2
Extracted molecule genomic DNA
Extraction protocol Protein-DNA complexes were isolated by antibody
Libraries were prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) (Cells Signaling Technology) and Multiplex Oligos for Illumina Systems (Single Index Primers) (ChIP-seq, CUT&RUN) (Cells Signaling Technology) following the manufacturer’s protocol
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina NovaSeq 6000
Data processing Reads mapped to hg38 with BWA
Duplicates removed with PICARD
Bigwig files generated with deeptools
Assembly: hg38
Supplementary files format and content: bigwig
Submission date Oct 04, 2023
Last update date Apr 16, 2024
Contact name Rui Li
Organization name University of Massachusetts Medical School
Department MCCB
Street address 364 Plantation Street, LRB
City Worcester
State/province Massachusetts
ZIP/Postal code 01605
Country USA
Platform ID GPL24676
Series (2)
GSE244630 Global mapping of H3K27ac and RPB1 occupancy upon WNK463 treatment
GSE244670 WNK463 effect on JJN3 cells
BioSample SAMN37686126
SRA SRX21991762

Supplementary file Size Download File type/resource 232.5 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA

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