|
Status |
Public on Apr 16, 2024 |
Title |
JJN3-WNK463-6h-1 |
Sample type |
SRA |
|
|
Source name |
JJN3
|
Organism |
Homo sapiens |
Characteristics |
cell line: JJN3 cell type: multiple myeloma cell line genotype: WT treatment: WNK463 time: 6h
|
Treatment protocol |
Cells were treated with DMSO or WNK463 (5μM) for 2/6 h
|
Growth protocol |
JJN3 cells were cultured in 40% DMEM 40% IMDM, and 20% FBS in 37°C supplemented with 10% CO2
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol following manufacturer's protocol Novogene
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
A6 counts.strict.txt
|
Data processing |
OneStopRNAseq server Sequence reads were trimmed for adapter sequence/low-quality sequence using Trimmomatic Trimmed sequence reads were mapped to hg38 using STAR Read count extraction was performed using featureCounts Assembly: hg38 Supplementary files format and content: counts.strict.txt
|
|
|
Submission date |
Oct 05, 2023 |
Last update date |
Apr 16, 2024 |
Contact name |
Rui Li |
Organization name |
University of Massachusetts Medical School
|
Department |
MCCB
|
Street address |
364 Plantation Street, LRB
|
City |
Worcester |
State/province |
Massachusetts |
ZIP/Postal code |
01605 |
Country |
USA |
|
|
Platform ID |
GPL24676 |
Series (2) |
GSE244667 |
Transcriptomics changes upon WNK463 treatment in JJN3 cells |
GSE244670 |
WNK463 effect on JJN3 cells |
|
Relations |
BioSample |
SAMN37689278 |
SRA |
SRX21993380 |