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Sample GSM7823583 Query DataSets for GSM7823583
Status Public on Apr 16, 2024
Title JJN3-DMSO-2
Sample type SRA
 
Source name JJN3
Organism Homo sapiens
Characteristics cell line: JJN3
cell type: multiple myeloma cell line
genotype: WT
treatment: DMSO
time: control
Treatment protocol Cells were treated with DMSO or WNK463 (5μM) for 2/6 h
Growth protocol JJN3 cells were cultured in 40% DMEM 40% IMDM, and 20% FBS in 37°C supplemented with 10% CO2
Extracted molecule total RNA
Extraction protocol Trizol following manufacturer's protocol
Novogene
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description B0
counts.strict.txt
Data processing OneStopRNAseq server
Sequence reads were trimmed for adapter sequence/low-quality sequence using Trimmomatic
Trimmed sequence reads were mapped to hg38 using STAR
Read count extraction was performed using featureCounts
Assembly: hg38
Supplementary files format and content: counts.strict.txt
 
Submission date Oct 05, 2023
Last update date Apr 16, 2024
Contact name Rui Li
Organization name University of Massachusetts Medical School
Department MCCB
Street address 364 Plantation Street, LRB
City Worcester
State/province Massachusetts
ZIP/Postal code 01605
Country USA
 
Platform ID GPL24676
Series (2)
GSE244667 Transcriptomics changes upon WNK463 treatment in JJN3 cells
GSE244670 WNK463 effect on JJN3 cells
Relations
BioSample SAMN37689277
SRA SRX21993381

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA

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