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Sample GSM7823586 Query DataSets for GSM7823586
Status Public on Apr 16, 2024
Title JJN3-shWNK1-1
Sample type SRA
Source name JJN3
Organism Homo sapiens
Characteristics cell line: JJN3
cell type: multiple myeloma cell line
treatment: shWNK1
Treatment protocol Cells were transduced with NS or WNK1 shRNA
Growth protocol JJN3 cells were cultured in 40% DMEM 40% IMDM, and 20% FBS in 37°C supplemented with 10% CO2
Extracted molecule total RNA
Extraction protocol Trizol following manufacturer's protocol
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Description A46
Data processing OneStopRNAseq server
Sequence reads were trimmed for adapter sequence/low-quality sequence using Trimmomatic
Trimmed sequence reads were mapped to hg38 using STAR
Read count extraction was performed using featureCounts
Assembly: hg38
Supplementary files format and content: counts.strict.txt
Submission date Oct 05, 2023
Last update date Apr 16, 2024
Contact name Rui Li
Organization name University of Massachusetts Medical School
Department MCCB
Street address 364 Plantation Street, LRB
City Worcester
State/province Massachusetts
ZIP/Postal code 01605
Country USA
Platform ID GPL24676
Series (2)
GSE244668 Transcriptomics changes upon WNK1 knockdown in JJN3 cells
GSE244670 WNK463 effect on JJN3 cells
BioSample SAMN37689295
SRA SRX21993384

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA

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