|
| Status |
Public on Jun 28, 2024 |
| Title |
ARN, Input2 |
| Sample type |
SRA |
| |
|
| Source name |
0h After Pupae Formation imaginal whole leg disc cells
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell type: control cells (whole leg disc cells)
time: 0h After Pupae Formation
genotype: R32D05-lexA>lexAopGFP
|
| Treatment protocol |
cell dissociation : collagenase + papain
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Zymo Quick-RNA microprep
NEBNext Ultra RNA library prep kit for illumina
oligodT
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
Input2
RPKM_all.txt
|
| Data processing |
Quality score trimming and filtering were performed with cutadapt.
reads were aligned to the annotated Dm6 genome (Ensembl release 70) using Hisat2 (v2.1.0)
Per gene read counts were calculated from the Hisat2 output using htseq-count (v0.11.2)
Differential gene expression was analyzed using the R package DESeq2 (v1.22.2)
Assembly: BDGP dm6
Supplementary files format and content: tab-delimited text file includes FBgn number, gene symbols and RPKM values for each sample.
|
| |
|
| Submission date |
Oct 12, 2023 |
| Last update date |
Jun 28, 2024 |
| Contact name |
yoan renaud |
| E-mail(s) |
yoan.renaud@uca.fr
|
| Organization name |
igred
|
| Street address |
place henri dunand
|
| City |
clermont ferrand |
| ZIP/Postal code |
63000 |
| Country |
France |
| |
|
| Platform ID |
GPL19132 |
| Series (1) |
| GSE245192 |
Transcriptomic analysis of Drosophila leg disc associated myoblasts |
|
| Relations |
| BioSample |
SAMN37799488 |
| SRA |
SRX22079832 |
