|
| Status |
Public on Dec 05, 2011 |
| Title |
HFD1a |
| Sample type |
RNA |
| |
|
| Source name |
Liver, high fat diet (HFD)
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
tissue: liver
strain: C57BL/6J
|
| Treatment protocol |
For NMN treatment, NMN was intraperitoneally given to HFD-mice for seven days.
|
| Growth protocol |
Mice were maintained on a regular chow or high fat diet.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from frozen liver samples with QIAGEN RNeasy kit in accordance with the prescribed protocol provided with the kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Chip-A
|
| Data processing |
The average of redundant genes were calculated in raw microarray results and data were subjected to Z score transformation, and Z ratio were calculated. False discovery rates for the differences in expression levels between two conditions (RC vs HFDa or HFDb vs NMN) were calculated using the Rank Product Analysis. All data were analyzed by the R statistical software package.
|
| |
|
| Submission date |
Aug 25, 2011 |
| Last update date |
Sep 15, 2023 |
| Contact name |
Jun Yoshino |
| E-mail(s) |
jyoshino@med.shimane-u.ac.jp
|
| Organization name |
Faculty of Medicine, Shimane University
|
| Street address |
89-1 Enya-cho
|
| City |
Izumo |
| State/province |
Shimane |
| ZIP/Postal code |
693-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE31647 |
Nicotinamide mononucleotide, a key NAD+ precursor, treats the pathophysiology of diet- and age-induced diabetes |
|
