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Sample GSM788394 Query DataSets for GSM788394
Status Public on Dec 31, 2011
Title WT-Root-+Zn-2
Sample type RNA
 
Source name WT_Root_+10 µM Zn2+ medium
Organism Arabidopsis halleri
Characteristics genotype: Langelsheim wild-type
age: 5 weeks old
tissue: Root
Extracted molecule total RNA
Extraction protocol RNA was extracted from homogenized A. halleri root tissue with Trizol (Invitrogen Life Technologies) according to the manufacturer’s instructions.
Label biotin
Label protocol 250 ng of total RNA, purified with RNeasy Mini columns (Qiagen, Hilden, Germany) according to the manufacturer's directions, were used to generate double-stranded cDNA and subsequently biotin-labeled cRNA. The length of the cRNA products was assessed using an Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, USA)
 
Hybridization protocol cRNA products were hybridized to the array for 16 h at 45C in a rotating chamber.
Scan protocol Hybridized arrays were washed and stained in an Affymetrix Fluidics Station FS450, and the fluorescent signals were measured with an Affymetrix GeneChip Scanner 3000.
Description Hydroponically grown 5-weeks old plants
Data processing CEL files from the Affymetrix microarray hybridization were processed by use of the R program and Bioconductor packages (Gentleman et al., 2004). The Robust Multi-chip Average (RMA) normalization was performed using the default settings of the corresponding R function (Irizarry et al., 2003). To estimate the amount of expressed mRNAs, the present call information of the non-parametric Wilcoxon signed rank test (PMA values) was computed with the 'affy' package (Gautier et al., 2004). Hybridization data from three biological replicates were generated for each Langelsheim wild-type and RNAi line 1-2.
 
Submission date Aug 31, 2011
Last update date Aug 15, 2018
Contact name Stephan Clemens
E-mail(s) stephan.clemens@uni-bayreuth.de
Organization name University of Bayreuth
Department Plant Physiology
Street address Universitaetsstrasse 30
City Bayreuth
ZIP/Postal code 95440
Country Germany
 
Platform ID GPL198
Series (1)
GSE31778 AhNAS2 suppression in Arabidopsis halleri roots
Relations
Reanalyzed by GSE118579

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
244901_at 5.6585952
244902_at 5.735449786
244903_at 7.664115681
244904_at 5.344868461
244905_at 4.148145416
244906_at 4.650744044
244907_at 3.640582106
244908_at 3.488411557
244909_at 3.592608888
244910_s_at 3.878137432
244911_at 3.032917432
244912_at 8.784117396
244913_at 4.479048908
244914_at 3.311174019
244915_s_at 4.07932307
244916_at 3.741643764
244917_at 3.839257598
244918_at 3.240250089
244919_at 6.624522932
244920_s_at 6.091104149

Total number of rows: 22810

Table truncated, full table size 490 Kbytes.




Supplementary file Size Download File type/resource
GSM788394_A645_02_Lang_W_Zn_2.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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