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Sample GSM788400 Query DataSets for GSM788400
Status Public on Dec 31, 2011
Title RNAi-Root-+Zn-2
Sample type RNA
Source name RNAi_Root_+10 µM Zn2+ medium
Organism Arabidopsis halleri
Characteristics genotype: AhNAS2-RNAi-1-2
age: 5 weeks old
tissue: Root
Extracted molecule total RNA
Extraction protocol RNA was extracted from homogenized A. halleri root tissue with Trizol (Invitrogen Life Technologies) according to the manufacturer’s instructions.
Label biotin
Label protocol 250 ng of total RNA, purified with RNeasy Mini columns (Qiagen, Hilden, Germany) according to the manufacturer's directions, were used to generate double-stranded cDNA and subsequently biotin-labeled cRNA. The length of the cRNA products was assessed using an Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, USA)
Hybridization protocol cRNA products were hybridized to the array for 16 h at 45C in a rotating chamber.
Scan protocol Hybridized arrays were washed and stained in an Affymetrix Fluidics Station FS450, and the fluorescent signals were measured with an Affymetrix GeneChip Scanner 3000.
Description Hydroponically grown 5-weeks old plants
Data processing CEL files from the Affymetrix microarray hybridization were processed by use of the R program and Bioconductor packages (Gentleman et al., 2004). The Robust Multi-chip Average (RMA) normalization was performed using the default settings of the corresponding R function (Irizarry et al., 2003). To estimate the amount of expressed mRNAs, the present call information of the non-parametric Wilcoxon signed rank test (PMA values) was computed with the 'affy' package (Gautier et al., 2004). Hybridization data from three biological replicates were generated for each Langelsheim wild-type and RNAi line 1-2.
Submission date Aug 31, 2011
Last update date Aug 15, 2018
Contact name Stephan Clemens
Organization name University of Bayreuth
Department Plant Physiology
Street address Universitaetsstrasse 30
City Bayreuth
ZIP/Postal code 95440
Country Germany
Platform ID GPL198
Series (1)
GSE31778 AhNAS2 suppression in Arabidopsis halleri roots
Reanalyzed by GSE118579

Data table header descriptions
VALUE RMA signal intensity

Data table
244901_at 5.923021471
244902_at 5.850768458
244903_at 7.20399603
244904_at 5.130199695
244905_at 3.870723595
244906_at 4.344215352
244907_at 3.347118455
244908_at 3.405921217
244909_at 3.576888702
244910_s_at 3.958275048
244911_at 3.05133076
244912_at 8.566657074
244913_at 4.288171994
244914_at 3.119960877
244915_s_at 4.116024227
244916_at 3.710449882
244917_at 3.660468976
244918_at 3.101500423
244919_at 5.596248267
244920_s_at 5.862743625

Total number of rows: 22810

Table truncated, full table size 490 Kbytes.

Supplementary file Size Download File type/resource
GSM788400_A645_05_RNAi_W_Zn_2.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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