|
| Status |
Public on Oct 28, 2005 |
| Title |
fasted liver standard chow diet 1 |
| Sample type |
RNA |
| |
|
| Source name |
liver
|
| Organism |
Rattus norvegicus |
| Characteristics |
liver sample of Wistar rat fed for 12 weeks with standard chow diet, after overnight fast
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Wistar rats were fasted overnight and killed by CO2. A piece of the reight venreal liver lobe was removed and clamp frozen. Total RNA was isolated from the clamp-frozen liver pieces using RNeasyR mini spin columns (Qiagen). The purified RNA was routinely checked for visible signs of degradation in a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, USA), and only high quality RNA was processed.
|
| Label |
biotin
|
| Label protocol |
Approximately 3-5 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
liver sample of Wistar rat fed for 12 weeks with standard chow diet, after overnight fast
|
| Data processing |
Affymetrix Microarray Suite version 5.0
|
| |
|
| Submission date |
Oct 20, 2005 |
| Last update date |
Oct 26, 2005 |
| Contact name |
Roland Buettner |
| E-mail(s) |
roland.buettner@klinik.uni-r.de
|
| Phone |
0049-941-944-7003
|
| Fax |
0049-041-944-7004
|
| Organization name |
University of Regensburg
|
| Department |
Internal Medicine I
|
| Lab |
Endocrinology
|
| Street address |
Franz-Josef-Strauss-Allee 11
|
| City |
Regensburg |
| ZIP/Postal code |
93042 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE3512 |
Fat-type specific effects of high fat diets |
|
