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Sample GSM7898124 Query DataSets for GSM7898124
Status Public on Dec 01, 2023
Title Lung 4 scRNA seq Multi
Sample type SRA
 
Source name lung
Organism Rattus norvegicus
Characteristics tissue: lung
Extracted molecule total RNA
Extraction protocol Freshly collected lungs were extracted. Half of one lobe was dissociated using the Mouse Lung Dissociation kit (Miltenyi Biotec) with the gentleMACS Octo Dissociator following the manufacurer’s recommendation.
Four samples were pooled and labeled using 10X Genomics CellPlex (PN-1000261) before loading onto Chip G and the Chromium Controller. Single Cell Libraries were generated using Next GEM Single Cell 3’ reagents v3.1 with dual indexing (10X Genomics). Library quality was assessed with a High Sensitivity DNA Chip on a BioAnalyzer (Agilent). Sequencing was done on a NovaSeq at MedGenome (Foster City, CA), 500M Paired-End Reads per Library.
 
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing the demultiplexing, barcorded processing, gene counting and aggregation were made using the cell ranger software v2.1.1
Supplementary files format and content: mm10
Supplementary files format and content: tab-separated values files and matrix files
 
Submission date Nov 13, 2023
Last update date Dec 01, 2023
Contact name Stefanos Zafeiropoulos
E-mail(s) szafeirop@gmail.com
Phone 5168591935
Organization name Feinstein Institutes for Medical Research
Street address 21 Walnut Road
City Glen Cove
State/province New York
ZIP/Postal code 11542
Country USA
 
Platform ID GPL25947
Series (1)
GSE247625 Gene expression profie at single cell level of lung cells in healthy rats and rats with pulmonary hypertension treated with sham-stimulation or ultrasound
Relations
BioSample SAMN38235911
SRA SRX22512720

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA

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