|
| Status |
Public on Sep 08, 2011 |
| Title |
VisCortex_S421A_rep1_scC |
| Sample type |
RNA |
| |
|
| Source name |
P17 visual cortex
|
| Organism |
Mus musculus |
| Characteristics |
strain: P17
litter: 1
genotype/variation: MeCP2 S421A
|
| Treatment protocol |
Mice were sacrificed and visual cortex dissected. All mice were male littermates from one of two litters.
|
| Growth protocol |
P17 mice (WT and MeCP2 S421A littermates) were grown under standard housing conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated by extraction using Trizol (invitrogen) according to the manufactures instructions, and then further purfied using qiagen minelute columns.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100ng total RNA
|
| |
|
| Hybridization protocol |
2 ug of Fragmented and Biotinylated cDNA were hybridized for 16 hr at 45C on Mouse Gene 1.0 ST microarray. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Genechip Scanner 3000
|
| Description |
P17 visual cortex RNA
|
| Data processing |
Data were processed using Affymetrix Expression Console Version 1.1 RMA workflow, quantile normalization and general background correction. Differential expression analysis was performed using GeneSpring GX (Agilent technologies) and custom statistical analysis see methods of Cohen et al. neuron 2011 for details.
|
| |
|
| Submission date |
Sep 02, 2011 |
| Last update date |
Sep 08, 2011 |
| Contact name |
Harrison Wren Gabel |
| E-mail(s) |
harrison_gabel@hms.harvard.edu
|
| Phone |
617-512-0289
|
| Organization name |
Harvard Medical School
|
| Department |
Neurobiology
|
| Lab |
Michael Greenberg
|
| Street address |
7 Linden St Apt 3
|
| City |
Brookline |
| State/province |
MA |
| ZIP/Postal code |
02445 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE31850 |
Genome-wide activity-dependent MeCP2 phosphorylation regulates nervous system development and function [visual cortex] |
| GSE31851 |
Genome-wide activity-dependent MeCP2 phosphorylation regulates nervous system development and function |
|
