|
| Status |
Public on Dec 01, 2023 |
| Title |
ASO1 rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Deceased female individual
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
cell type: Primary hepatocyte cultures
|
| Treatment protocol |
Hepatocytes in 12 well plates were treated for 72 hwith 60 nM final of ASO1, ASO2 or a Non-target ASO.
|
| Growth protocol |
William's E, 10% serum, pen/strep and hepatocyte growth supplements (ThermoFisher)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
High Pure RNA isolation using Trizol and cartridges
|
| Label |
Biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Samples were hybridized to a Clariom_D array (Affymetrix) and scanned at the Centre for Applied Genomics (TCAG), Toronto
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Data processing |
Relative expression changes assessed by Expression Console
|
| |
|
| Submission date |
Nov 29, 2023 |
| Last update date |
Dec 01, 2023 |
| Contact name |
Ruth McPherson |
| E-mail(s) |
rmcpherson@ottawaheart.ca
|
| Organization name |
UOHI
|
| Street address |
40 Ruskin
|
| City |
Ottawa |
| State/province |
Ontario |
| ZIP/Postal code |
K1Y4W7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE248931 |
TRIBAL/TRIBA1AL suppression in human hepatocytes |
|
