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Sample GSM7992700 Query DataSets for GSM7992700
Status Public on Dec 26, 2023
Title sgRNA_Spl2
Sample type SRA
Source name Tissue Resident Memory T cells
Organism Mus musculus
Characteristics infection: 14 dpi of LCMV
mouse replicate: 1
tissue: Spleen
Extracted molecule total RNA
Extraction protocol Cells were isolated from frozen, flow cytometry sorted CD8+ T cell subsets according to the sort markers noted in the per-sample descriptions
Perturb-seq libraries were constructed using 10x Genomics reagents and protocols for 5' feature barcoding and gene expression
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Data processing Raw reads for gene expression were processed using CellRanger v6 for the mm10 reference transcriptome
sgRNA counts were computed using kallisto | bustools for a custom kite index of gRNAs present in the library
Assembly: mm10
Supplementary files format and content: h5 of gene expression counts matrices; barcodes/genes/counts for sgRNA content from CRISPR perturbation
Submission date Dec 26, 2023
Last update date Dec 27, 2023
Contact name Caleb Lareau
Organization name Memorial Sloan Kettering
Street address 417 E 68th St, Zuckerman - ZRC 1132
City New York
State/province New York
ZIP/Postal code 10065
Country USA
Platform ID GPL24247
Series (2)
GSE243597 A unique epigenomic landscape defines CD8+ tissue-resident memory T cells
GSE252041 A unique epigenomic landscape defines CD8+ tissue-resident memory T cells [Perturb-seq]
BioSample SAMN39129437
SRA SRX23030063

Supplementary file Size Download File type/resource
GSM7992700_sgRNA_Spl2_counts.barcodes.txt.gz 313.7 Kb (ftp)(http) TXT
GSM7992700_sgRNA_Spl2_counts.genes.txt.gz 262 b (ftp)(http) TXT
GSM7992700_sgRNA_Spl2_counts.mtx.gz 513.0 Kb (ftp)(http) MTX
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