|
| Status |
Public on Nov 06, 2012 |
| Title |
Med20_Dcr1_vs_Dcr1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
med20 and dcr1 double mutant fission yeast cells
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
genotype/variation: med20 and dcr1 double mutant
|
| Growth protocol |
Yeast cells were grown to mid-log phase in YEA rich medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from mid-log phase cultures according to Xuefeng zhu et al. 2006 . cDNA converted from RNA.
|
| Label |
Biotin
|
| Label protocol |
Labeling was performed according to the GeneChip expression analysis technical manual that is available for download at www.affymetrix.com.
|
| |
|
| Channel 2 |
| Source name |
Dcr1 mutant yeast cells
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
genotype/variation: Dcr1 mutant
|
| Growth protocol |
Yeast cells were grown to mid-log phase in YEA rich medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from mid-log phase cultures according to Xuefeng zhu et al. 2006 . cDNA converted from RNA.
|
| Label |
Biotin
|
| Label protocol |
Labeling was performed according to the GeneChip expression analysis technical manual that is available for download at www.affymetrix.com.
|
| |
|
| |
| Hybridization protocol |
According to the GeneChip expression analysis technical manual that is available for download at www.affymetrix.com.
|
| Scan protocol |
According to the GeneChip expression analysis technical manual that is available for download at www.affymetrix.com.
|
| Description |
Med20_Dcr1_A
Med20_Dcr1_B
cDNA from Dcr1 mutnat Biological Rep 1-2, chip A
cDNA from Dcr1 mutnat Biological Rep 1-2, chip B
|
| Data processing |
Raw data (CEL files) was analyzed in affymetrix tilling array analysis software,The raw cel data were imported into TAS as two analysis treatment group -- dblmutant Vs mutant. two side test, bandwidth 150 . Probe match chosen was PM/MM(perfect match/mismatch). Finally, we analyzed the intensity to get the Bar format signal files.
|
| |
|
| Submission date |
Sep 22, 2011 |
| Last update date |
Nov 06, 2012 |
| Contact name |
Claes M Gustafsson |
| E-mail(s) |
claes.gustafsson@medkem.gu.se
|
| Organization name |
Gothenburg University
|
| Department |
Department of Medical biochemistry and cell biology
|
| Street address |
Medcinaregatan 9A
|
| City |
Gothenburg |
| ZIP/Postal code |
405 30 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL7715 |
| Series (2) |
| GSE32310 |
Transcriptome analysis of mediator Med20 mutant and dcr1 mutant in S.pombe |
| GSE35527 |
Mediator promotes CENP-A incorporation at fission yeast centromeres |
|
