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Sample GSM801092 Query DataSets for GSM801092
Status Public on Jan 01, 2012
Title ECs from EC/NSPC co-culture, incubated with PD 173074 2
Sample type other
 
Source name Mouse primary brain endothelial cells (Celprogen) grown in co-culture with neural stem/progenitor cells isolated from E14 mouse embryonic brain
Organism Mus musculus
Characteristics cell type: primary brain endothelial cells (Celprogen, San Diego, CA, catalog # 11053-07)
sample type: ECs isolated from the mouse forebrain microvessels and NSPCs from telencephalon of E-14 mouse embryo
treatment: EC/NSPC co-culture treated with FGF/VEGF receptor inhibitor
Treatment protocol co-cultures were treated with : PD 173074 (1mM in DMSO), SB431542 (10μM in DMSO), or 2μl/mL DMSO (control), for 24 hours.
Growth protocol ECs were grown in transwell co-culture with NSPCs,in growth factor-free NSPC medium with addition of 5% FBS, at 37C, in CO2 incubator
Extracted molecule other
Extraction protocol After 24 hours in transwell co-culture, the ECs and NSPCs were separated and miRNA s were isolated using mirVana miRNA (AB/Ambion) isolation kit
Label biotin
Label protocol FlashTag RNA Labeling with Genisphere RNA Labeling Kit
 
Hybridization protocol Following labeling, 21.5 ul of biotin-labeled RNA was hybridized for 16 hr at 48C on Affymetrix miRNA 2.0 Arrays. The miRNA 2.0 Arrays were washed and stained in the Affymetrix Fluidics Station 450 using fludics script FS450_0003.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Description miRNA expression data from EC/NSPC co-culture treated with FGF/VEGF receptor inhibitor
Data processing Data files were analyzed with Affymetrix miRNAQCtool using RMA background correction and quantile normalization (Default settings)
 
Submission date Sep 23, 2011
Last update date Jan 01, 2012
Contact name Tamara Roitbak
E-mail(s) troitbak@salud.unm.edu, ggpickett@salud.unm.edu
Phone 505 272-3401
Fax 505 272-6091
Organization name UNM
Department Neurosurgery
Street address 2212 Lomas NE
City Albuquerque
State/province NM
ZIP/Postal code 87131
Country USA
 
Platform ID GPL14613
Series (1)
GSE32352 Role of microRNAs in Neural Stem Cell-supported Endothelial Morphogenesis

Data table header descriptions
ID_REF
VALUE log10 GC_RMA signal

Data table
ID_REF VALUE
mmu-let-7g_st 38.73481
mmu-let-7g-star_st 20.28481
mmu-let-7i_st 679.3842
mmu-let-7i-star_st 14.93082
mmu-miR-1_st 12.71165
mmu-miR-15b_st 118.1645
mmu-miR-15b-star_st 14.68594
mmu-miR-23b_st 1438.53
mmu-miR-27b-star_st 17.17018
mmu-miR-27b_st 119.4368
mmu-miR-29b-star_st 15.39887
mmu-miR-29b_st 21.71446
mmu-miR-30a_st 35.26671
mmu-miR-30a-star_st 15.89195
mmu-miR-30b_st 27.18547
mmu-miR-30b-star_st 19.98777
mmu-miR-99a_st 65.21126
mmu-miR-99b_st 1110.537
mmu-miR-99b-star_st 92.74436
mmu-miR-101a-star_st 15.08635

Total number of rows: 1412

Table truncated, full table size 37 Kbytes.




Supplementary file Size Download File type/resource
GSM801092.CEL.gz 621.5 Kb (ftp)(http) CEL
Processed data included within Sample table

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