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Status |
Public on Jun 05, 2013 |
Title |
24 imbibed dormant, biological rep1 |
Sample type |
RNA |
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|
Source name |
dormant seeds imbibed for 24 h
|
Organism |
Triticum aestivum |
Characteristics |
tissue: seed genotype: AC Domain age: mature
|
Treatment protocol |
One-half of the seeds were stored at -80oC to retain dormancy.To obtain AR seeds, the remaining portion of the seeds was stored at room temperature for 10 months.
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Growth protocol |
Wheat plants were grown in a greenhouse at 16-22oC/14-18oC (day/night) under a 16/8 h photoperiod until harvest. Spikes were harvested at maturity.
|
Extracted molecule |
total RNA |
Extraction protocol |
mRNA isolation using PolyATtract kit according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared using the GeneChip IVT Labelling Kit and the GeneChip Sample Cleanup Module.
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|
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Hybridization protocol |
Following fragmentation, labelled cRNA samples were hybridized for 16 hr at 45C on GeneChip Wheat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using an Affymetrix Scanner 3000.
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Description |
Gene expression data from dormant seeds imbibed for 24 h.
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Data processing |
The data were analyzed with Robust Multi-array Average methodology (RMA).
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|
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Submission date |
Sep 27, 2011 |
Last update date |
Jun 05, 2013 |
Contact name |
Belay T. Ayele |
Organization name |
University of Manitoba
|
Department |
Plant Science
|
Lab |
Crop Technology Center
|
Street address |
222 Agriculture Building
|
City |
Winnipeg |
State/province |
MB |
ZIP/Postal code |
R3T 2N2 |
Country |
Canada |
|
|
Platform ID |
GPL3802 |
Series (1) |
GSE32409 |
Expression data from dormant and after-ripened wheat seeds and after-ripened seeds imbibed in ABA. |
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