|
| Status |
Public on Jun 05, 2013 |
| Title |
24 imbibed dormant, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
dormant seeds imbibed for 24 h
|
| Organism |
Triticum aestivum |
| Characteristics |
tissue: seed
genotype: AC Domain
age: mature
|
| Treatment protocol |
One-half of the seeds were stored at -80oC to retain dormancy.To obtain AR seeds, the remaining portion of the seeds was stored at room temperature for 10 months.
|
| Growth protocol |
Wheat plants were grown in a greenhouse at 16-22oC/14-18oC (day/night) under a 16/8 h photoperiod until harvest. Spikes were harvested at maturity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA isolation using PolyATtract kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the GeneChip IVT Labelling Kit and the GeneChip Sample Cleanup Module.
|
| |
|
| Hybridization protocol |
Following fragmentation, labelled cRNA samples were hybridized for 16 hr at 45C on GeneChip Wheat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix Scanner 3000.
|
| Description |
Gene expression data from dormant seeds imbibed for 24 h.
|
| Data processing |
The data were analyzed with Robust Multi-array Average methodology (RMA).
|
| |
|
| Submission date |
Sep 27, 2011 |
| Last update date |
Jun 05, 2013 |
| Contact name |
Belay T. Ayele |
| Organization name |
University of Manitoba
|
| Department |
Plant Science
|
| Lab |
Crop Technology Center
|
| Street address |
222 Agriculture Building
|
| City |
Winnipeg |
| State/province |
MB |
| ZIP/Postal code |
R3T 2N2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL3802 |
| Series (1) |
| GSE32409 |
Expression data from dormant and after-ripened wheat seeds and after-ripened seeds imbibed in ABA. |
|
