 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Feb 29, 2024 |
| Title |
TDP_3 |
| Sample type |
SRA |
| |
|
| Source name |
whole insect
|
| Organism |
Triatoma dimidiata |
| Characteristics |
tissue: whole insect
population: Lambda-cyhalothrin pressure population
time: Day 3
|
| Growth protocol |
The insects were obtained when they were three days old, ensuring their color and weight were homogeneous, and stored at -80°C until processing
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed using the Quick-RNA Miniprep kit (Zymo Research, USA), and DNA traces were removed by adding DNase I, following the manufacturer's instructions.
The library preparation was performed using the TruSeq Stranded LT Sample Preparation Kit (Illumina, USA), following the manufacturer's instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Trimmage trimmomatic-0.39.jar
Trinity --seqType fq
Alignment-free abundance estimation methods SALMON
Assembly: de novo
Supplementary files format and content: MATRIX file included raw counts for each sample
|
| |
|
| Submission date |
Jan 25, 2024 |
| Last update date |
Feb 29, 2024 |
| Contact name |
Sara Zuluaga |
| E-mail(s) |
sarazuluagaa@gmail.com
|
| Organization name |
Universidad de Antioquia
|
| Department |
Antioquia
|
| Lab |
BCEI
|
| Street address |
CL 62 #52-59
|
| City |
Medellín |
| ZIP/Postal code |
050010 |
| Country |
Colombia |
| |
|
| Platform ID |
GPL34132 |
| Series (1) |
| GSE254270 |
New point mutations in the sodium channel gene and deciphering pyrethroid resistance using RNA-seq in Triatoma dimidiata from Colombia |
|
| Relations |
| BioSample |
SAMN39613986 |
| SRA |
SRX23396806 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
