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Sample GSM8037227 Query DataSets for GSM8037227
Status Public on Feb 18, 2024
Title Nat10_gKO_3
Sample type SRA
Source name oocyte
Organism Mus musculus
Characteristics cell type: oocyte
genotype: Gdf9-cre Nat10 cKO
Extracted molecule polyA RNA
Extraction protocol The fully growing oocytes (FGO) were harvested from WT and Gdf9-cre Nat10 conditional knockout female mice 36-48h after PMSG injection, then after washing by PBS with 0.2% containing, the oocytes were lysed with lysis buffer containing 0.2% Triton X-100 and RRI.
The library construction were standard Smart-seq2 protocol. Briefly, polyA RNA in cell lysis were first hybrided with Oligo-dT primer, then reverse transcripted by the PrimeScript Ⅱ RTase and TSO primer. The producted cDNA were pre-amplified with KAPA HiFi HotStart Ready Mix (2×) & TSO-PCR primer. Purified intact cDNA were then fragmented and amplified with barcodes adding by using TruePrep DNA Lybrary Prep Kit V2 for Illumina (cat.#TD502, Vazyme).
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
Data processing The raw reads were first cleaned with Trim Galore v0.6.7, and the cleaned reads were further mapped to mm10 genome by using STAR v2.7.10a. Uniquely mapped reads were applied for gene expression quantification by using FeatureCounts b2.0.2. The trimmed reads were also mapped to the ERCC genome for gaining the percentage of ERCC.
Assembly: mm10
Supplementary files format and content: The count list calibrated by ERCC.
Submission date Jan 26, 2024
Last update date Feb 18, 2024
Contact name Heng-yu Fan
Organization name Life Sciences Institute
Lab Fan heng-yu
Street address Yuhangtang Road
City Hangzhou
State/province Zhejiang
ZIP/Postal code 310058
Country China
Platform ID GPL18480
Series (1)
GSE254288 NAT10-mediated mRNA N4-acetylation is Essential for the Translational Regulation During Oocyte Meiotic Maturation in Mice
BioSample SAMN39620385
SRA SRX23402501

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA

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