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Sample GSM8080509 Query DataSets for GSM8080509
Status Public on Feb 17, 2024
Title ccRCC with PC destruction 1
Sample type RNA
Source name renal cell carcinoma
Organism Rattus norvegicus
Characteristics sample type: i-Cap 2-3 (PC destruction)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with miRneasy FFPE (QIAGEN, Venlo, Netherland)Kit followed by DNase I treatment.
Label SYBR Green
Label protocol PCR assays were performed using RT2 Profiler PCR Array (QIAGEN,Venlo, Netherland) , Rat Tumor Metastasis <PARN-028-ZD> , following the Manufacturer’s instructions. Reverse transcription was performed from 500 ng total RNA using the RT2 First Strand Kit (QIAGEN, Venlo, Netherland). Quantitative real-time PCR were performed (Light Cycler 480, Roche) 40 cycles of denaturation at 95˚C for 15 s; annealing and a final extension at 60˚C for 1 min.
Hybridization protocol n/a
Scan protocol n/a
Description Test
Data processing The normalization and all the data analysis were performed according to the manufacturers instructions using their web-based software package:
For the normalization it uses the average of five housekeeping genes: ACTB, B2M, HPRT1, LDHA, RPL1
Target gene signals normalized to housekeeping genes; 2^-deltaCt, where deltaCt = (Ct_Target − Ct_HKG)]
The web-based software package automatically performs all deltadeltaCt based fold-change calculations from the uploaded raw threshold cycle data.
Submission date Feb 14, 2024
Last update date Feb 17, 2024
Contact name Takuto Shimizu
Organization name Nara Medical University
Department Department of Urology
Street address 840 Shijo-cho
City Kashihara
State/province Nara
ZIP/Postal code 6348522
Country Japan
Platform ID GPL34195
Series (1)
GSE255820 Real-time quantitative PCR analysis of ccRCC model rats II

Data table header descriptions
VALUE Fold Change worksheet reports test group average/control group average(i.e., i-Cap2-3/i-Cap1) ratios.

Data table
A01 0.188363927
A02 0.188363927
A03 0.188363927
A05 1.266658538
A11 0.200107727
B01 0.188363927
B02 0.188363927
B03 0.33876342
B04 0.188363927
B05 0.188363927
B06 1.249767418
B07 0.33929213

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided

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