NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM8083016 Query DataSets for GSM8083016
Status Public on Apr 18, 2024
Title Levels of miRNAs in the A549 Ago2-KO/HA-Ago2Wt cells, 3
Sample type RNA
 
Source name miRNA from A549 Ago2-KO/HA-Ago2Wt cells
Organism Homo sapiens
Characteristics cell line: A549
genotype: Ago2-KO, and OE HA-Ago2Wt cells
Treatment protocol A549 Ago2-KO/HA-Ago2Wt, Ago2-KO/HA-Ago2KA, and Ago2-KO/HA-Ago2Δ (Dm) cells were collected and total RNA (included miRNA) werw extracted by Trizol reagent.
Growth protocol A549 Ago2-KO/HA-Ago2Wt, Ago2-KO/HA-Ago2KA, and Ago2-KO/HA-Ago2Δ (Dm) cells were seeded into 15-cm dishes (2 × 106 cells per dish) and incubated for 3 days.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated miRNA were prepared using FlashTag Biotin HSR RNA Labeling Kit.
 
Hybridization protocol 21.5 μLof Biotin-labeled sample were hybridized for 16 hr at 48℃ on miRNA 3.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 7G.
Description miRNA expression data from A549 cells
Data processing Affymetrix GeneChip Command Console software (version4.0, Affymetrix) was used to analyze array images to get raw data and then Affymetrix Expression Console 1.3.1. for normalization; Partek Genomics Suite software for statistical analysis
 
Submission date Feb 16, 2024
Last update date Apr 18, 2024
Contact name YI-JU CHEN
E-mail(s) iii895830@gmail.com
Organization name National Health Research Institutes
Street address Room R1-3121, 35 keyan Road, Zhunan Town
City Miaoli County
ZIP/Postal code 35053
Country Taiwan
 
Platform ID GPL19117
Series (1)
GSE255941 miRNA array in the A549 Ago2-KO/HA-Ago2Wt, Ago2-KO/HA-Ago2KA, and Ago2-KO/HA-Ago2Δ (Dm) cells

Data table header descriptions
ID_REF
VALUE rma-dabg.chp Signal

Data table
ID_REF VALUE
MIMAT0000062_st 12.52
MIMAT0004481_st 0.77
MIMAT0010195_st 1.51
MIMAT0000063_st 12.8
MIMAT0004482_st 1.22
MIMAT0000064_st 12.05
MIMAT0026472_st 0.18
MIMAT0000065_st 11.69
MIMAT0004484_st 3.31
MIMAT0000066_st 12.47
MIMAT0004485_st 2.57
MIMAT0000067_st 9.25
MIMAT0004486_st 0.54
MIMAT0004487_st 0.54
MIMAT0000068_st 7.94
MIMAT0004488_st 0.29
MIMAT0000069_st 12.27
MIMAT0004489_st 0.04
MIMAT0000070_st 11.32
MIMAT0000071_st 5.13

Total number of rows: 6631

Table truncated, full table size 133 Kbytes.




Supplementary file Size Download File type/resource
GSM8083016_W_3.CEL.gz 722.6 Kb (ftp)(http) CEL

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap