|
| Status |
Public on Apr 12, 2024 |
| Title |
tdTomato-positive CD4-positive cell from splenocytes of SM22alpha-Cre reporter mouse 2 |
| Sample type |
SRA |
| |
|
| Source name |
Spleen
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Spleen
cell type: CD4+ T cell
cell subset: tdTomato-positive
genotype: Ai14; SM22alpha-Cre
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells and tissues were lysed in Trizol and RNA was extracted using PureLink RNA Mini Kit (Thermo Fisher Scientific,) and PureLink DNase (Thermo Fisher Scientific,) according to manufacturer’s instructions.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
FACS
Spleen CD4 of SM22a-Cre reporter mice.csv
|
| Data processing |
A minimum 25M reads per library with 75 bp paired-ends reads were generated per sample.
Quality control of the resulting sequence data was performed using CLC genomics workbench
Trimmed and filtered reads were aligned to rat genome Rn6 reference and mouse genome GRCm38
Assembly: Rn6 and GRCm38
Supplementary files format and content: Matrix table with FPKM values for every gene and every sample
|
| |
|
| Submission date |
Feb 16, 2024 |
| Last update date |
Apr 12, 2024 |
| Contact name |
Tadakatsu Inagaki |
| E-mail(s) |
inagaki.tadakatsu@ncvc.go.jp
|
| Organization name |
National Cerebral and Cardiovascular Center
|
| Department |
Vascular Physiology
|
| Street address |
6-1, Kishibesinnmachi
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
564-8565 |
| Country |
Japan |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE255994 |
IL-6/gp130 signaling in CD4+ T cells drives the pathogenesis of pulmonary hypertension |
|
| Relations |
| BioSample |
SAMN39969337 |
| SRA |
SRX23645962 |
