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Sample GSM8083933 Query DataSets for GSM8083933
Status Public on Apr 12, 2024
Title tdTomato-negative CD4-positive cell from splenocytes of SM22alpha-Cre reporter mouse 3
Sample type SRA
Source name Spleen
Organism Mus musculus
Characteristics tissue: Spleen
cell type: CD4+ T cell
cell subset: tdTomato-negative
genotype: Ai14; SM22alpha-Cre
Extracted molecule total RNA
Extraction protocol Cells and tissues were lysed in Trizol and RNA was extracted using PureLink RNA Mini Kit (Thermo Fisher Scientific,) and PureLink DNase (Thermo Fisher Scientific,) according to manufacturer’s instructions.
RNA libraries were prepared for sequencing using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Description FACS
Spleen CD4 of SM22a-Cre reporter mice.csv
Data processing A minimum 25M reads per library with 75 bp paired-ends reads were generated per sample.
Quality control of the resulting sequence data was performed using CLC genomics workbench
Trimmed and filtered reads were aligned to rat genome Rn6 reference and mouse genome GRCm38
Assembly: Rn6 and GRCm38
Supplementary files format and content: Matrix table with FPKM values for every gene and every sample
Submission date Feb 16, 2024
Last update date Apr 12, 2024
Contact name Tadakatsu Inagaki
Organization name National Cerebral and Cardiovascular Center
Department Vascular Physiology
Street address 6-1, Kishibesinnmachi
City Suita
State/province Osaka
ZIP/Postal code 564-8565
Country Japan
Platform ID GPL19057
Series (1)
GSE255994 IL-6/gp130 signaling in CD4+ T cells drives the pathogenesis of pulmonary hypertension
BioSample SAMN39969333
SRA SRX23645966

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA

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