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Sample GSM8098257 Query DataSets for GSM8098257
Status Public on Feb 26, 2024
Title pos_ctl+pid 1-X per treatment/timepoint group rep 4
Sample type SRA
Source name PBMCs
Organism Macaca fascicularis
Characteristics tissue: PBMCs
genotype: WT
treatment: pos_ctl
Treatment protocol AAV9 peptide pool (3 mg/mL/peptide final concentration) or DMSO (negative control) or anti-CD3 (positive control) were added, and cells were cultured for approximately 24 hours at 37oC with 5% CO2
Growth protocol PBMCs from immunized monkeys were thawed and washed with CTL Anti-Aggregate WashTM Solution (ImmunoSpot/Cellular Technology Limited), resuspended in CTL-TestTM Media (ImmunoSpot/Cellular Technology Limited), counted, and evaluated for viability
Extracted molecule total RNA
Extraction protocol RNA isolation was performed using the Qiagen RNeasy Lipid Tissue (Qiagen, #74804) protocol on the Qiacubes according to manufacturer’s instruction
25 ng RNA was used as input for the Lexogen QuantSeq 3’mRNA-Seq Library Preparation kit for Illumina (Forward ) (Lexogen, #015.96) and libraries were prepared according to the manufacturer’s protocol
3' bulk RNAseq
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Description pid
Data processing aligned sample reads to Ensembl Macaca_fascicularis_6.0 using Salmon v 14.0
Assembly: Crab-eating macaque (Macaca_fascicularis_6.0)
Supplementary files format and content: txt file
Submission date Feb 22, 2024
Last update date Feb 26, 2024
Contact name Richard Virgen-Slane
Organization name Pfizer
Department Global Computational Safety Science, DSRD
Street address 0777 Science Center Drive, CB4-2131, Pfizer La Jolla
City San Diego
State/province CA
ZIP/Postal code 92121
Country USA
Platform ID GPL22523
Series (1)
GSE256418 Cell-mediated Immune Response Signature to AAV Capsid in Cynomolgus Monkeys
BioSample SAMN40043271
SRA SRX23708368

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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