|
| Status |
Public on Feb 26, 2024 |
| Title |
dmso+nec 1-X per treatment/timepoint group rep 1 |
| Sample type |
SRA |
| |
|
| Source name |
PBMCs
|
| Organism |
Macaca fascicularis |
| Characteristics |
tissue: PBMCs
genotype: WT
treatment: dmso
|
| Treatment protocol |
AAV9 peptide pool (3 mg/mL/peptide final concentration) or DMSO (negative control) or anti-CD3 (positive control) were added, and cells were cultured for approximately 24 hours at 37oC with 5% CO2
|
| Growth protocol |
PBMCs from immunized monkeys were thawed and washed with CTL Anti-Aggregate WashTM Solution (ImmunoSpot/Cellular Technology Limited), resuspended in CTL-TestTM Media (ImmunoSpot/Cellular Technology Limited), counted, and evaluated for viability
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation was performed using the Qiagen RNeasy Lipid Tissue (Qiagen, #74804) protocol on the Qiacubes according to manufacturer’s instruction
25 ng RNA was used as input for the Lexogen QuantSeq 3’mRNA-Seq Library Preparation kit for Illumina (Forward ) (Lexogen, #015.96) and libraries were prepared according to the manufacturer’s protocol
3' bulk RNAseq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
nec
|
| Data processing |
aligned sample reads to Ensembl Macaca_fascicularis_6.0 using Salmon v 14.0
Assembly: Crab-eating macaque (Macaca_fascicularis_6.0)
Supplementary files format and content: txt file
|
| |
|
| Submission date |
Feb 22, 2024 |
| Last update date |
Feb 26, 2024 |
| Contact name |
Richard Virgen-Slane |
| E-mail(s) |
Richard.Virgen-Slane@pfizer.com
|
| Organization name |
Pfizer
|
| Department |
Global Computational Safety Science, DSRD
|
| Street address |
0777 Science Center Drive, CB4-2131, Pfizer La Jolla
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
| |
|
| Platform ID |
GPL22523 |
| Series (1) |
| GSE256418 |
Cell-mediated Immune Response Signature to AAV Capsid in Cynomolgus Monkeys |
|
| Relations |
| BioSample |
SAMN40043265 |
| SRA |
SRX23708377 |
