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Sample GSM8106146 Query DataSets for GSM8106146
Status Public on Feb 27, 2024
Title TF ChIP-seq from whole organism (ENCLB849KLH)
Sample type SRA
 
Source name Drosophila melanogaster strain Pdp1-MiMIC-GFP mixed sex embryo (0-24 hours) insertion targeting D. melanogaster Pdp1
Organism Drosophila melanogaster
Characteristics antibody: Pdp1
cell_type: whole organism
biomaterial_type: whole organisms
biosample encode accession: ENCBS008URC (SAMN40086048)
collection date: not applicable
geographic location: not applicable
encode accession: ENCBS008URC
note: D. mel line contraining an intronic eGFP exon to create a Pdp1-GFP fusion gene
Sex: pooled male and female
dev stage: embryonic
age: 0-24 hour
donor_id: ENCDO034JNF
lab: Kevin White, UChicago
source: Hugo Bellen
link: ENCBS008URC at ENCODE; https://www.encodeproject.org/ENCBS008URC/
Extracted molecule genomic DNA
Extraction protocol general protocol: https://www.encodeproject.org/documents/47d3fb40-f2e5-427a-9e29-501dfdaf41fb/@@download/attachment/modENCODE_protocols_Column_SPRI.pdf
other: https://www.encodeproject.org/documents/745e29b2-2b52-496d-be43-2dca31a07a29/@@download/attachment/1277.pdf
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2000
 
Description https://www.encodeproject.org/experiments/ENCSR005MLL/
***************
biological replicate number: 3
technical replicate number: 1
description: ChIP-seq on transgenic flies expressing Pdp1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
experiment encode accession: ENCSR005MLL
assay title: TF ChIP-seq
assembly: dm6, dm3
possible controls: ENCSR546LJI
encode release date: 2016-07-06
lab: Kevin White, UChicago
library encode accession: ENCLB849KLH
fragmentation methods: sonication (Bioruptor generic)
size range: 200-800
Data processing See GSM*_README.txt supplementary file linked below
 
Submission date Feb 24, 2024
Last update date Feb 27, 2024
Contact name ENCODE DCC
E-mail(s) encode-help@lists.stanford.edu
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
 
Platform ID GPL13304
Series (1)
GSE256558 TF ChIP-seq from whole organism (ENCSR005MLL)
Relations
BioSample SAMN40086048

Supplementary file Size Download File type/resource
GSM8106146_ENCFF078TQL_peaks_and_background_as_input_for_IDR_dm6.bigBed 1.2 Mb (ftp)(http) BIGBED
GSM8106146_ENCFF078YNT_read-depth_normalized_signal_dm6.bigWig 16.5 Mb (ftp)(http) BIGWIG
GSM8106146_ENCFF146DOY_control_normalized_signal_dm3.bigWig 15.2 Mb (ftp)(http) BIGWIG
GSM8106146_ENCFF177DIR_control_normalized_signal_dm6.bigWig 16.5 Mb (ftp)(http) BIGWIG
GSM8106146_ENCFF206RZQ_signal_of_unique_reads_dm6.bigWig 16.5 Mb (ftp)(http) BIGWIG
GSM8106146_ENCFF445JLD_peaks_and_background_as_input_for_IDR_dm3.bed.gz 733.0 Kb (ftp)(http) BED
GSM8106146_ENCFF502NUM_peaks_and_background_as_input_for_IDR_dm3.bigBed 1.2 Mb (ftp)(http) BIGBED
GSM8106146_ENCFF568PIJ_peaks_and_background_as_input_for_IDR_dm6.bed.gz 732.7 Kb (ftp)(http) BED
GSM8106146_ENCFF832DIG_signal_of_unique_reads_dm3.bigWig 14.5 Mb (ftp)(http) BIGWIG
GSM8106146_ENCFF920CDI_read-depth_normalized_signal_dm3.bigWig 14.5 Mb (ftp)(http) BIGWIG
GSM8106146_ENCLB849KLH_README.txt.gz 1.9 Kb (ftp)(http) TXT
Raw data are available in SRA

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