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Sample GSM8152094 Query DataSets for GSM8152094
Status Public on Mar 21, 2024
Title hepatoid gland adenoma (HGA), 8 [h29]
Sample type RNA
 
Source name archival FFPE tumour tissue
Organism Canis lupus familiaris
Characteristics tissue: FFPE tumour tissue
animal: female, neutered
breed: Australian Shepherd
dv200 (%): 74.2
age (years): 11
Extracted molecule total RNA
Extraction protocol Total RNA was extracted utilising the PureLinkTM FFPE Total RNA Isolation Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA).
Label not provided
Label protocol not provided
 
Hybridization protocol 150 to 250 ng of total RNA was hybridised to the nCounter Canine IO Panel XT CodeSets (NanoString Technologies, Inc., Seattle, WA, USA). Hybridised samples were processed following the manufacturer’s protocols (protocol ID: MAN-10023-11, MAN-10056-06) and loaded onto the nCounter MAX Analysis System’s (NanoString) Prep Station for purification and immobilization on sample cartridges.
Scan protocol Samples were transferred to the nCounter MAX Digital Analyzer (NanoString) for data collection following the manufacturer’s user manual (protocol ID: MAN-C0035-08).
Description h29
Data processing Following the workflow described in the manufacturer’s user manuals (MAN-C0019-08, MAN-C0011-04), the reporter library files (RLF) and reporter code count (RCC) files were imported into the nSolver 4.0 Analysis Software. Quality control and normalisation followed default settings.
 
Submission date Mar 18, 2024
Last update date Mar 21, 2024
Contact name Alexander F. H. Haake
E-mail(s) alexander.haake@fu-berlin.de
Organization name Freie Universität Berlin
Department Department of Veterinary Medicine
Lab Institute of Veterinary Pathology
Street address Robert-von-Ostertag-Straße 15
City Berlin
ZIP/Postal code 14163
Country Germany
 
Platform ID GPL34305
Series (1)
GSE261791 Strong Correlation of Gene Counts and Differentially Expressed Genes between a 3′ RNA-Seq and RNA Hybridisation Platform from Canine Archival Tissue [nCounter]

Data table header descriptions
ID_REF
VALUE normalized

Data table
ID_REF VALUE
1 20064.00
2 6979.00
3 2028.00
4 420.00
5 72.00
6 38.00
7 1.00
8 4.00
9 10.00
10 5.00
11 5.00
12 13.00
13 8.00
14 2.00
15 34.72
16 252.40
17 32.05
18 301.81
19 385.95
20 900.09

Total number of rows: 844

Table truncated, full table size 8 Kbytes.




Supplementary file Size Download File type/resource
GSM8152094_20220203_AH04_417-116_11.RCC.gz 9.5 Kb (ftp)(http) RCC

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