|
| Status |
Public on Oct 01, 2013 |
| Title |
Leaves from Ca. L. asiaticus infected plant 6, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Citrus leaves from Ca. L. asiaticus infected plants, 8 months after innoculation
|
| Organism |
Citrus sinensis |
| Characteristics |
tissue: old symptomatic leaves
age: ~2 year old plants graft innoculated with Ca. L. asiaticus
infection: Candidatus Liberibacter asiaticus
|
| Treatment protocol |
Valencia sweet orange (C. sinensis) plants were graft inoculated with budwood from Ca. L. asiaticus-infected citrus plants
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from freshly obtained leaf tissues using RNeasy Plant Mini kit and treated with DNase (Qiagen, Valencia, CA) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Four micrograms of total RNA was used to generate biotinylated cRNA using one-cycle target labelling kit (Affymetrix, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 450C on GeneChip Citrus Genome Array. GeneChips were washed and stained in the Affymetrix Model 450 Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using Affymetrics Model 3000 7G scanner according to the manufacture's recommended protocols.
|
| Description |
Valencia sweet orange
Gene expression data in leaves infected with Ca. L. asiaticus
|
| Data processing |
Raw data collected using Microarray Suite version 5.0 (MAS 5.0). Statistical tests were performed using the BioConductor statistical software, Version 2.5.
Normalsied log2 fold change (leaves from Ca. L. asiaticus infected plants/healthy); False discovery rate; detection P-values; MAS5.0 signal intensity
|
| |
|
| Submission date |
Oct 14, 2011 |
| Last update date |
Oct 01, 2013 |
| Contact name |
Valente Aritua |
| E-mail(s) |
varitua@ufl.edu
|
| Phone |
863-956-1151
|
| Organization name |
University of Florida
|
| Department |
Plant Pathology
|
| Street address |
700 Experiment Staion Road
|
| City |
Lake Alfred |
| State/province |
Florida |
| ZIP/Postal code |
33850 |
| Country |
USA |
| |
|
| Platform ID |
GPL5731 |
| Series (1) |
| GSE33003 |
Response of Sweet Orange (Citrus sinensis) to Candidatus Liberibacter asiaticus Infection |
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