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Sample GSM8195463 Query DataSets for GSM8195463
Status Public on Apr 12, 2024
Title Cytotrophoblast infected, biol rep 3
Sample type SRA
 
Source name Trophoblast cells
Organisms Toxoplasma gondii; Homo sapiens
Characteristics tissue: Trophoblast cells
cell line: Trophoblast stem cell (clone 27)
cell type: Cytotrophoblast
treatment: Trophoblast stem cell media
Growth protocol Trophoblast stem cells were seeded at a ratio of 1.5 x 10^5 in 6-well plates and induced to form syncytiotrophoblast cells using a differentiation medium. After 5 days of differentiation, cells were infected or mock-infected with T. gondii RH: YFP (5 parasites/cell). For comparison, cytotrophoblast cells were seeded in a 6-well plate and infected or mock-infected with T. gondii at the same MOI used for syncytiotrophoblast cells.
Extracted molecule polyA RNA
Extraction protocol Qiagen Rneasy
RNA libraries for RNA-seq were prepared using NEBNext Ultra II Directional RNA Library Prep Kit following manufacturer's protocols.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model NextSeq 2000
 
Description RS_06_S6
Data processing CLC Genomics Workbench v 23.0
Assembly: Read libraries were mapped to the human (hg38) transcriptomes using CLC Genomics Workbench.
Supplementary files format and content: tab-delimited text files include raw counts for each sample
 
Submission date Apr 09, 2024
Last update date Apr 19, 2024
Contact name Rafaela Jose da Silva
E-mail(s) rjs224@pitt.edu
Phone (412) 214-3649
Organization name University of Pittburgh
Department Biological Sciences
Street address 4249 Fifth Avenue
City Pittsburgh
State/province Pennsylvania
ZIP/Postal code 15260
Country USA
 
Platform ID GPL34372
Series (1)
GSE263615 Transcriptome analysis of cytotrophoblasts and syncytiotrophoblasts derived from trophoblast stem cells, mock-infected or infected with Toxoplasma gondii
Relations
BioSample SAMN40906530
SRA SRX24203190

Supplementary file Size Download File type/resource
GSM8195463_CYT_infected_3_raw_counts.txt.gz 2.3 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA

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