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Sample GSM8200271 Query DataSets for GSM8200271
Status Public on Apr 16, 2024
Title clec-35_biol rep 3^
Sample type SRA
 
Source name Whole animal
Organism Caenorhabditis elegans
Characteristics tissue: Whole animal
genotype: clec-35(icb105)
treatment: L4 stage_20°C
Treatment protocol Nematode Growth Media agar plates supplemented with E. coli OP50 at 20°C
Growth protocol All worm strains were prepared by bleaching gravid adults to isolate embyros which were grown until L4 stage where extract (or water control) treatment was performed for 4 hours before collecting for RNA isolation.
Extracted molecule total RNA
Extraction protocol TRIzol (Life Technologies)
By Novogene
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing Reads were aligned to C. elegans genome WS283
Reads were counted using Kallisto
Transcript abundance was quantified using the Sleuth and Wald test
Assembly: WormBase Release WS283
Supplementary files format and content: H5 files having raw TPM counts for all genes
 
Submission date Apr 12, 2024
Last update date Apr 16, 2024
Contact name Michalis Barkoulas
E-mail(s) m.barkoulas@imperial.ac.uk
Organization name Imperial College London
Street address SAF Building, South Kensington
City London
ZIP/Postal code SW7 2AZ
Country United Kingdom
 
Platform ID GPL26672
Series (1)
GSE263858 Transcriptomic analysis of C. elegans mutants which do not respond to oomycete exposure
Relations
BioSample SAMN40945387
SRA SRX24235635

Supplementary file Size Download File type/resource
GSM8200271_clec-35_biol_rep_3__abundance.h5 14.8 Mb (ftp)(http) H5
SRA Run SelectorHelp
Raw data are available in SRA

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