|
Status |
Public on Apr 16, 2024 |
Title |
clec-35_biol rep 3^ |
Sample type |
SRA |
|
|
Source name |
Whole animal
|
Organism |
Caenorhabditis elegans |
Characteristics |
tissue: Whole animal genotype: clec-35(icb105) treatment: L4 stage_20°C
|
Treatment protocol |
Nematode Growth Media agar plates supplemented with E. coli OP50 at 20°C
|
Growth protocol |
All worm strains were prepared by bleaching gravid adults to isolate embyros which were grown until L4 stage where extract (or water control) treatment was performed for 4 hours before collecting for RNA isolation.
|
Extracted molecule |
total RNA |
Extraction protocol |
TRIzol (Life Technologies) By Novogene
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
Reads were aligned to C. elegans genome WS283 Reads were counted using Kallisto Transcript abundance was quantified using the Sleuth and Wald test Assembly: WormBase Release WS283 Supplementary files format and content: H5 files having raw TPM counts for all genes
|
|
|
Submission date |
Apr 12, 2024 |
Last update date |
Apr 16, 2024 |
Contact name |
Michalis Barkoulas |
E-mail(s) |
m.barkoulas@imperial.ac.uk
|
Organization name |
Imperial College London
|
Street address |
SAF Building, South Kensington
|
City |
London |
ZIP/Postal code |
SW7 2AZ |
Country |
United Kingdom |
|
|
Platform ID |
GPL26672 |
Series (1) |
GSE263858 |
Transcriptomic analysis of C. elegans mutants which do not respond to oomycete exposure |
|
Relations |
BioSample |
SAMN40945387 |
SRA |
SRX24235635 |