|
| Status |
Public on Apr 15, 2024 |
| Title |
Pancreas, KC, replicate4 |
| Sample type |
SRA |
| |
|
| Source name |
pancreas
|
| Organism |
Mus musculus |
| Characteristics |
tissue: pancreas
cell type: epithelial and immune cells
genotype: KC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Single-cell suspensions were prepared by mincing murine pancreas followed by digestion with Collagenase P (4 mg/mL), Dispase (4mg/mL) and DNAse (0.5 mg/mL) and incubation for 5-10 minutes at 37°C in a shaker. Samples were then filtered through a 70-μm and 40-μm strainer and stained with L/D aqua (Thermo Fisher) at Room temperature for 10 minutes. Samples were resuspended in PBS with 0.004% bovine serum albumin and sorted for live cells using Aria II sorter. Cell viability was measured using Trypan Blue. Single-cell suspensions were loaded in a 10x Genomics (Pleasanton, CA) Chromium instrument to generate gel beads in emulsion.
The raw data was processed using cell ranger count (Cell Ranger v2.1.1; 10x Genomics) based on the mm10 mouse reference genome
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
10X Genomics
|
| Data processing |
The raw data was processed using cell ranger count (Cell Ranger v2.1.1; 10x Genomics) based on the mm10 mouse reference genome
Assembly: mm10
Supplementary files format and content: Tab-separated value files and matrix files
|
| |
|
| Submission date |
Apr 15, 2024 |
| Last update date |
Aug 13, 2024 |
| Contact name |
Lourdes Susana Castro Pando |
| E-mail(s) |
susanacp26@gmail.com, lscastro@mdanderson.org
|
| Organization name |
UT MD ANDERSON CANCER CTR.
|
| Street address |
7777 Greenbriar Drive, Apt 2050
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030-4530 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE264054 |
Pancreatic epithelial IL-17/IL-17RA signaling drives B7-H4 to promote tumorigenesis |
|
| Relations |
| BioSample |
SAMN40975133 |
| SRA |
SRX24268338 |
