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Sample GSM8209364 Query DataSets for GSM8209364
Status Public on Apr 15, 2024
Title Pancreas, KC, replicate4
Sample type SRA
 
Source name pancreas
Organism Mus musculus
Characteristics tissue: pancreas
cell type: epithelial and immune cells
genotype: KC
Extracted molecule total RNA
Extraction protocol Single-cell suspensions were prepared by mincing murine pancreas followed by digestion with Collagenase P (4 mg/mL), Dispase (4mg/mL) and DNAse (0.5 mg/mL) and incubation for 5-10 minutes at 37°C in a shaker. Samples were then filtered through a 70-μm and 40-μm strainer and stained with L/D aqua (Thermo Fisher) at Room temperature for 10 minutes. Samples were resuspended in PBS with 0.004% bovine serum albumin and sorted for live cells using Aria II sorter. Cell viability was measured using Trypan Blue. Single-cell suspensions were loaded in a 10x Genomics (Pleasanton, CA) Chromium instrument to generate gel beads in emulsion.
The raw data was processed using cell ranger count (Cell Ranger v2.1.1; 10x Genomics) based on the mm10 mouse reference genome
 
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description 10X Genomics
Data processing The raw data was processed using cell ranger count (Cell Ranger v2.1.1; 10x Genomics) based on the mm10 mouse reference genome
Assembly: mm10
Supplementary files format and content: Tab-separated value files and matrix files
 
Submission date Apr 15, 2024
Last update date Apr 15, 2024
Contact name Lourdes Susana Castro Pando
E-mail(s) susanacp26@gmail.com
Organization name UT MD ANDERSON CANCER CTR.
Street address 7777 Greenbriar Drive, Apt 2050
City Houston
State/province Texas
ZIP/Postal code 77030-4530
Country USA
 
Platform ID GPL19057
Series (1)
GSE264054 Pancreatic epithelial IL-17/IL-17RA signaling drives B7-H4 to promote tumorigenesis
Relations
BioSample SAMN40975133

Supplementary file Size Download File type/resource
GSM8209364_Filtered_sample_8.tar.gz 1.7 Mb (ftp)(http) TAR
Raw data are available in SRA

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