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Sample GSM8212808 Query DataSets for GSM8212808
Status Public on Apr 16, 2024
Title dpf61_Ov_mt_3
Sample type SRA
Source name Ovary
Organism Danio rerio
Characteristics tissue: Ovary
age: dpf 61
genotype: mutant
Growth protocol WT AB strain and mutant zebrafish lines were housed in the zebrafish core facility with a 14-hour(h) light and 10h dark photoperiod, lights on at 8:30am, lights off at 10:30pm; at water temperature of 28.5°C, pH of approximately 7.2, and salinity/conductivity ranging from 500 to 1,200µS in two automatically controlled standalone zebrafish rearing systems. Fish are fed to satiation three times daily with a commercial food.
Extracted molecule polyA RNA
Extraction protocol Ovary tissues were homogenized immediately in RNAzol RT reagent and stored at -800C till purification. Total RNA was purified following the manufacturer’s protocol
cDNA was made for three replicates for each time point by genotype with the Tecan Ovation RNAseq kit for 47 and 61dpf samples. Libraries were then prepared with the Tecan Celero EZ DNA-Seq library kit for 47 and 61dpf samples.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Data processing Data were pseudoaligned with Kallisto.
Assembly: GRCz11
Supplementary files format and content: tab-delimited file that has raw counts for each sample.
Submission date Apr 16, 2024
Last update date Apr 16, 2024
Contact name Ciro Maurizio Amato
Phone 2398257829
Organization name National Institute of Environmental Health Sciences
Department Reproductive and Developmental Biology Laboratory
Lab Reproductive Developmental Biology
Street address 111 Tw Alexander Dr
City Research Triangle Park
State/province NC
ZIP/Postal code 27709
Country USA
Platform ID GPL20828
Series (1)
GSE264143 Adamts9 Is Required for Development of Primary Ovarian Follicles and Maintenance of Female Sex in Zebrafish
BioSample SAMN40985262
SRA SRX24276192

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA

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