NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM822494 Query DataSets for GSM822494
Status Public on Jul 07, 2012
Title NPC case NPC28
Sample type RNA
 
Source name Nasopharyngeal carcinoma
Organism Homo sapiens
Characteristics tissue: NPC tumor
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using an acid phenol/chloroform extraction method followed by ethanol precipitation.
Label pCp-DY647
Label protocol 2.5 μg of total RNA was employed with 100 nmol/L of pCp-DY647 (Dharmacon, Lafayette, CO, USA) and 15 Units of T4 RNA ligase (USB, Cleveland, Oh, USA) in a total reaction volume of 20 µL at 16°C overnight
 
Hybridization protocol The labeled RNAs were hybridized to the array with a 1×hybridization solution (5× Denhart’s solution, 0.5% SDS, 5× SSC) in a Hybridization Chamber (Corning Inc, Corning, NY, USA) at 46°C for 12 to 16 hours.
Scan protocol Scanned in a LuxScanTM -10K Scanner (capitalBio Inc.,Beijing, China).
Data processing Quantile normalization across multiple arrays, log 2 transformation of expression levels, BRB ArrayTools v.3.8.0 was used.
 
Submission date Oct 25, 2011
Last update date Jul 07, 2012
Contact name bijun huang
E-mail(s) yanghj@sysucc.org.cn
Organization name Cancer Center, Sun Yat-Sen University, Guangzhou, China
Department State Key Laboratory of Oncology in Southern China
Lab 617
Street address 651#Dongfeng East Road
City Guangzhou
ZIP/Postal code 510060
Country China
 
Platform ID GPL14769
Series (1)
GSE33225 Epstein-Barr Virus-Encoded miRNAome Associated with Specific Latent Type in Tumor Cells

Data table header descriptions
ID_REF
VALUE quantile normalized log2 signal

Data table
ID_REF VALUE
29 13.04551328
30 9.16322951
31 13.85791619
32 9.696967526
33 13.08770217
34 9.983626397
35 9.33737437
36 12.74329174
37 9.759149174
38 11.70043972
39 9.45349906
40 12.15948602
41 10.05754098
42 9.656424863
43 9.794235518
44 12.44989379
45 9.878391232
46 9.360480604
47 9.409861857
48 9.785179434

Total number of rows: 923

Table truncated, full table size 14 Kbytes.




Supplementary file Size Download File type/resource
GSM822494_NPC28.gpr.gz 40.1 Kb (ftp)(http) GPR
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap