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Sample GSM822495 Query DataSets for GSM822495
Status Public on Jul 07, 2012
Title NPC cell line C666-1
Sample type RNA
 
Source name EBV positive NPC cell line
Organism Homo sapiens
Characteristics cell type: NPC cell line
cell line: C666-1
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using an acid phenol/chloroform extraction method followed by ethanol precipitation.
Label pCp-DY647
Label protocol 2.5 μg of total RNA was employed with 100 nmol/L of pCp-DY647 (Dharmacon, Lafayette, CO, USA) and 15 Units of T4 RNA ligase (USB, Cleveland, Oh, USA) in a total reaction volume of 20 µL at 16°C overnight
 
Hybridization protocol The labeled RNAs were hybridized to the array with a 1×hybridization solution (5× Denhart’s solution, 0.5% SDS, 5× SSC) in a Hybridization Chamber (Corning Inc, Corning, NY, USA) at 46°C for 12 to 16 hours.
Scan protocol Scanned in a LuxScanTM -10K Scanner (capitalBio Inc.,Beijing, China).
Data processing Quantile normalization across multiple arrays, log 2 transformation of expression levels, BRB ArrayTools v.3.8.0 was used.
 
Submission date Oct 25, 2011
Last update date Jul 07, 2012
Contact name bijun huang
E-mail(s) yanghj@sysucc.org.cn
Organization name Cancer Center, Sun Yat-Sen University, Guangzhou, China
Department State Key Laboratory of Oncology in Southern China
Lab 617
Street address 651#Dongfeng East Road
City Guangzhou
ZIP/Postal code 510060
Country China
 
Platform ID GPL14769
Series (1)
GSE33225 Epstein-Barr Virus-Encoded miRNAome Associated with Specific Latent Type in Tumor Cells

Data table header descriptions
ID_REF
VALUE quantile normalized log2 signal

Data table
ID_REF VALUE
29 13.64548326
30 10.27612441
31 9.721857942
32 10.05392588
33 13.78969217
34 9.541311551
35 9.545607419
36 13.04551328
37 9.582665134
38 12.33585564
39 9.574909467
40 13.2935195
41 9.620626729
42 9.401589938
43 9.589755998
44 10.69406793
45 9.365836862
46 9.652049031
47 9.57784798
48 9.639843529

Total number of rows: 923

Table truncated, full table size 14 Kbytes.




Supplementary file Size Download File type/resource
GSM822495_C666-1.gpr.gz 41.3 Kb (ftp)(http) GPR
Processed data included within Sample table

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