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Sample GSM822497 Query DataSets for GSM822497
Status Public on Jul 07, 2012
Title lymphocytic cell line Raji
Sample type RNA
Source name EBV positive lymphocytic cell line
Organism Homo sapiens
Characteristics cell type: lymphocytic cell line
cell line: Raji
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using an acid phenol/chloroform extraction method followed by ethanol precipitation.
Label pCp-DY647
Label protocol 2.5 μg of total RNA was employed with 100 nmol/L of pCp-DY647 (Dharmacon, Lafayette, CO, USA) and 15 Units of T4 RNA ligase (USB, Cleveland, Oh, USA) in a total reaction volume of 20 µL at 16°C overnight
Hybridization protocol The labeled RNAs were hybridized to the array with a 1×hybridization solution (5× Denhart’s solution, 0.5% SDS, 5× SSC) in a Hybridization Chamber (Corning Inc, Corning, NY, USA) at 46°C for 12 to 16 hours.
Scan protocol Scanned in a LuxScanTM -10K Scanner (capitalBio Inc.,Beijing, China).
Data processing Quantile normalization across multiple arrays, log 2 transformation of expression levels, BRB ArrayTools v.3.8.0 was used.
Submission date Oct 25, 2011
Last update date Jul 07, 2012
Contact name bijun huang
Organization name Cancer Center, Sun Yat-Sen University, Guangzhou, China
Department State Key Laboratory of Oncology in Southern China
Lab 617
Street address 651#Dongfeng East Road
City Guangzhou
ZIP/Postal code 510060
Country China
Platform ID GPL14769
Series (1)
GSE33225 Epstein-Barr Virus-Encoded miRNAome Associated with Specific Latent Type in Tumor Cells

Data table header descriptions
VALUE quantile normalized log2 signal

Data table
29 13.70234111
30 10.26951594
31 10.99717948
32 10.25016693
33 12.94767748
34 10.55703858
35 9.844095294
36 13.76800056
37 9.498516162
38 11.78418046
39 9.693680349
40 13.72639561
41 11.27120388
42 9.571331717
43 10.41268733
44 12.23873641
45 9.703519496
46 9.121820992
47 9.622661357
48 9.309603678

Total number of rows: 923

Table truncated, full table size 14 Kbytes.

Supplementary file Size Download File type/resource
GSM822497_Raji.gpr.gz 39.2 Kb (ftp)(http) GPR
Processed data included within Sample table

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