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Sample GSM822499 Query DataSets for GSM822499
Status Public on Jul 07, 2012
Title lymphocytic cell line Namalwa
Sample type RNA
 
Source name EBV positive lymphocytic cell line
Organism Homo sapiens
Characteristics cell type: lymphocytic cell line
cell line: Namalwa
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using an acid phenol/chloroform extraction method followed by ethanol precipitation.
Label pCp-DY647
Label protocol 2.5 μg of total RNA was employed with 100 nmol/L of pCp-DY647 (Dharmacon, Lafayette, CO, USA) and 15 Units of T4 RNA ligase (USB, Cleveland, Oh, USA) in a total reaction volume of 20 µL at 16°C overnight
 
Hybridization protocol The labeled RNAs were hybridized to the array with a 1×hybridization solution (5× Denhart’s solution, 0.5% SDS, 5× SSC) in a Hybridization Chamber (Corning Inc, Corning, NY, USA) at 46°C for 12 to 16 hours.
Scan protocol Scanned in a LuxScanTM -10K Scanner (capitalBio Inc.,Beijing, China).
Data processing Quantile normalization across multiple arrays, log 2 transformation of expression levels, BRB ArrayTools v.3.8.0 was used.
 
Submission date Oct 25, 2011
Last update date Jul 07, 2012
Contact name bijun huang
E-mail(s) yanghj@sysucc.org.cn
Organization name Cancer Center, Sun Yat-Sen University, Guangzhou, China
Department State Key Laboratory of Oncology in Southern China
Lab 617
Street address 651#Dongfeng East Road
City Guangzhou
ZIP/Postal code 510060
Country China
 
Platform ID GPL14769
Series (1)
GSE33225 Epstein-Barr Virus-Encoded miRNAome Associated with Specific Latent Type in Tumor Cells

Data table header descriptions
ID_REF
VALUE quantile normalized log2 signal

Data table
ID_REF VALUE
29 10.56330211
30 9.211348095
31 9.868822555
32 9.368264173
33 10.53613937
34 12.26206224
35 9.50205805
36 7.541739391
37 10.03189277
38 9.600738066
39 10.4969646
40 7.80949805
41 12.23873641
42 9.442713393
43 9.564783984
44 8.854868383
45 10.09627635
46 8.610615176
47 9.83025057
48 8.587882504

Total number of rows: 923

Table truncated, full table size 14 Kbytes.




Supplementary file Size Download File type/resource
GSM822499_Namalwa.gpr.gz 42.3 Kb (ftp)(http) GPR
Processed data included within Sample table

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