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Sample GSM822501 Query DataSets for GSM822501
Status Public on Jul 07, 2012
Title lymphocytic cell line Akata
Sample type RNA
 
Source name EBV positive lymphocytic cell line
Organism Homo sapiens
Characteristics cell type: lymphocytic cell line
cell line: Akata(+)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using an acid phenol/chloroform extraction method followed by ethanol precipitation.
Label pCp-DY647
Label protocol 2.5 μg of total RNA was employed with 100 nmol/L of pCp-DY647 (Dharmacon, Lafayette, CO, USA) and 15 Units of T4 RNA ligase (USB, Cleveland, Oh, USA) in a total reaction volume of 20 µL at 16°C overnight
 
Hybridization protocol The labeled RNAs were hybridized to the array with a 1×hybridization solution (5× Denhart’s solution, 0.5% SDS, 5× SSC) in a Hybridization Chamber (Corning Inc, Corning, NY, USA) at 46°C for 12 to 16 hours.
Scan protocol Scanned in a LuxScanTM -10K Scanner (capitalBio Inc.,Beijing, China).
Data processing Quantile normalization across multiple arrays, log 2 transformation of expression levels, BRB ArrayTools v.3.8.0 was used.
 
Submission date Oct 25, 2011
Last update date Jul 07, 2012
Contact name bijun huang
E-mail(s) yanghj@sysucc.org.cn
Organization name Cancer Center, Sun Yat-Sen University, Guangzhou, China
Department State Key Laboratory of Oncology in Southern China
Lab 617
Street address 651#Dongfeng East Road
City Guangzhou
ZIP/Postal code 510060
Country China
 
Platform ID GPL14769
Series (1)
GSE33225 Epstein-Barr Virus-Encoded miRNAome Associated with Specific Latent Type in Tumor Cells

Data table header descriptions
ID_REF
VALUE quantile normalized log2 signal

Data table
ID_REF VALUE
29 9.62469312
30 9.127277308
31 12.007767
32 9.230421596
33 10.27819156
34 8.700439718
35 9.71672345
36 9.885019093
37 9.318165423
38 9.307833771
39 9.52160044
40 9.180461188
41 8.909560116
42 9.331414119
43 10.18280773
44 9.671245598
45 10.30644112
46 8.625303371
47 9.313134724
48 8.564785889

Total number of rows: 923

Table truncated, full table size 14 Kbytes.




Supplementary file Size Download File type/resource
GSM822501_Akata.gpr.gz 38.5 Kb (ftp)(http) GPR
Processed data included within Sample table

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