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Sample GSM8257307 Query DataSets for GSM8257307
Status Public on May 10, 2024
Title S2-WT-1
Sample type SRA
Source name S2 cell
Organism Drosophila melanogaster
Characteristics cell line: S2 cell
genotype: WT
Growth protocol Cells grown at 27 degrees celcius, in Schneider's Insect Cell Media, supplemented with Fetal Bovine Serum and antibotics (Penicillin and Streptomycin)
Extracted molecule total RNA
Extraction protocol RNA was extracted using TRIzol. The upper phase was taken, and RNA was precipitated. Precpitated RNA was dissolved in dH2O.
Library was constructed by Novogene, following polyA enrichment
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq X Plus
Description instrument model: Illumina NovaSeq X Plus
Data processing Sequences trimmed using TRIMMOMATIC PE
Sequences aligned to Dm6.56 using HISAT2
SAMtools to sort and convert sam files to BAM files
HTSeq to calculate raw counts from bam files
DESeq2 for differential expression analysis
Assembly: Dm6.56 FlyBase (
Supplementary files format and content: Raw gene counts in plain .txt files, annotated with FlyBase Gene ID
Submission date May 07, 2024
Last update date May 10, 2024
Contact name Matthew Wright
Organization name University of Birmingham
Department School of Biosciences
Street address Edgbaston
City Birmingham
ZIP/Postal code B15 2TT
Country United Kingdom
Platform ID GPL34457
Series (1)
GSE266138 UPF1 is required for gene expression in mitochondria and for the elimination of paternal mtDNA
BioSample SAMN41253845
SRA SRX24485335

Supplementary file Size Download File type/resource
GSM8257307_HD1_genes.txt.gz 63.7 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA

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