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Sample GSM828661 Query DataSets for GSM828661
Status Public on Jun 18, 2012
Title Keratinocytes_I482T_rep2
Sample type RNA
Source name Primary neonatal keratinocytes
Organism Homo sapiens
Characteristics cell type: primary neonatal keratinocytes
genotype/variation: ΔNp63α(I482T) Overexpression
Treatment protocol siRNA knock down
Growth protocol Keratinocytes were grown in Gibco SFM medium till 100% confluent with addition of 1.2 mM Ca2+ for 3 Days to induce differentiation.
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kits were used to isolate total RNA.
Label biotin
Label protocol Affymetrix 3' IVT Express Kit
Hybridization protocol Affymetrix GeneChip Hybridization, Wash, and Stain Kit
Scan protocol Affymetrix GeneChip Scanner 3000 7G
Description replicate 2
Data processing RMA normalization using justRMA package in R 2.11.1
Submission date Nov 04, 2011
Last update date Oct 11, 2022
Contact name Douglas Porter
Organization name Stanford
Department Dermatology
Lab Khavari
Street address 269 Campus Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platform ID GPL570
Series (2)
GSE33495 Disrupted transcripitonal network in ΔNp63 AEC tissue model [gene expression]
GSE33572 Disrupted transcripitonal network in ΔNp63 AEC tissue model

Data table header descriptions
VALUE RNA-normalized value

Data table
1007_s_at 11.0807115
1053_at 7.156581901
117_at 5.205774206
121_at 7.434655276
1255_g_at 3.464897909
1294_at 5.329706659
1316_at 5.479267262
1320_at 5.462466077
1405_i_at 3.827655578
1431_at 3.888317369
1438_at 7.065021196
1487_at 8.197245219
1494_f_at 5.960320446
1552256_a_at 8.732251772
1552257_a_at 10.02448059
1552258_at 5.113672647
1552261_at 4.380633449
1552263_at 7.242994901
1552264_a_at 7.997957576
1552266_at 3.329995731

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.

Supplementary file Size Download File type/resource
GSM828661.CEL.gz 4.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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